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CJAP ›› 2019, Vol. 35 ›› Issue (2): 165-168.doi: 10.12047/j.cjap.5729.2019.036

• ORIGINAL ARTICLES • Previous Articles     Next Articles

Effects of Notch signal on the expressions of HIF-α and autophagy- related genes Beclin1, LC3I, LC3II in oxygen-glucose deprivation induced myocardial cell injury

KONG Ling-yu 1, XI Zan 2, MA Wen-ting 3, YANG Fei-yun 1, NIU Li-dan 1, SHI Jin-he1△   

  1. 1. Department of Emergency, the First Affiliated Hospital of Xinxiang Medical University, Weihui 453100;
    2. Department of Pediatric Surgery, the First Affiliated Hospital of Xinxiang Medical University, Weihui 453100;
    3. Department of Diagnostic Laboratory of Sanquan College, Xinxiang Medical University, Xinxiang 453003, China
  • Online:2019-03-28 Published:2019-06-27

Abstract: Objective: To investigate the effects of Notch signal on hypoxic induction factor (HIF-1α) and autophagy-associated genes Beclin1, LC3I, LC3II in oxygen-glucose deprivation (OGD) induced myocardial cell injury. Methods: The OGD model was established using hypoxic culture box and hypoglycemic DMEM medium. The cells were divided into normal control group, OGD group, OGD + NC siRNA group, OGD + Notch1 siRNA group and OGD + HIF-1α siRNA group. Western blot was used to detect the interference effects of HIF-1α siRNA and Notch1 siRNA. The effects of Notch1 siRNA and HIF-1α siRNA on the activity of myocardial cells in OGD model were detected by the CCK-8 assay. The effects of Notch1 siRNA and HIF-1α siRNA on autophage-associated genes Beclin1, LC3I and LC3II expression were detected by Western blot. Results: The results of Western blot showed that HIF-1α siRNA could effectively knock down the expression of HIF-1α in myocardial cells in OGD model, and Notch1 siRNA could effectively knock down the expression of Notch1 and HIF-1α in myocardial cells in OGD model. The result of CCK-8 assay showed that Notch1 siRNA and HIF-1α siRNA reduced the activity of myocardial cells in OGD model, and there was no statistical difference between the two groups. Western blot results showed that Notch1 siRNA and HIF-1α siRNA could reduce the expressions of the autophagy-associated genes Beclin1, LC3I and LC3II, and reduce the ratio of LC3II to LC3I at mRNA level. Conclusion: Notch1 plays a role in myocardial protection by regulating the expression of HIF-1α to regulate the autophagy in OGD model cells.

Key words: Notch signaling pathway, hypoxic induction factor, ischemia and hypoxia, autophagy

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