The intervention of ginkgo biloba extract on monocrotaline-induced right ventricular hypertrophy in rats and its mechanism

doi:10.12047/j.cjap.5759.2019.037

Abstract

Abstract: Objective: To study the protective effects of ginkgo biloba extract on the right ventricular hypertrophy. Methods: Seventy-two SD male rats were randomly divided into 3 groups: control group(CON), monocrotaline-induced right ventricular hypertrophy group (MCT) and ginkgo biloba extract treated group (EGB) (n=24 in each group). Group MCT and group EGB were intraperitoneally injected with 2%MCT at the dose of 60 mg /kg on the first day. From the second day, group MCT was injected with 2 ml 0.9% sodium chloride, and 60 mg/kg ginkgo leaf extract was administered to the stomach in group EGB. The control group was injected with 2 ml 0.9% sodium chloride on the first day. After 3 weeks, in each group,cardiac hemodynamic changes were measured, heart weight index was calculated, and myocardial pathological changes were observed by HE staining. The expression of TRPC6 was detected by real-time polymerase chain reaction (real-time -PCR) and Western blot. Results: Compared with the control group, the right ventricular systolic pressure (RVSP) was increased significantly in the MCT group(P＜0.01), the maximum or decline rate of descent (RV±dp/dt_max) of the right ventricle pressure was increased significantly(P＜0.01), while the EGB group had the same trend as all the indexes in the group MCT, but the amplitude of all indicators in group EGB were decreased significantly than those of group MCT(P＜0.01), and the right ventricular hypertrophy index (RVMI) in group EGB was significantly lower than that in group MCT(P＜0.01).Group MCT showed typical myocardial hypertrophy performance by HE staining, and the right ventricular myocytes in group EGB were significantly improved than that in group MCT, and the mRNA and protein expression levels of TRPC6 in the right ventricle of group MCT and group EGB were increased(P＜0.01), while the EGB group was significantly lower than that of the MCT group(P＜0.01). Conclusion: Ginkgo biloba extract may inhibit the signal pathway of CaN / NFAT in cardiac myocytes by reducing the expression of TRPC6 protein, and then play an early protective effect on myocardial hypertrophy.

Key words: ginkgo biloba extract, rat, monocrotaline, transient receptor potential channels, cardiac hypertrophy

CHEN Hui-qin, CAI Ke-feng. The intervention of ginkgo biloba extract on monocrotaline-induced right ventricular hypertrophy in rats and its mechanism[J]. CJAP, 2019, 35(2): 169-172.

References

[1] 高继康. 慢性肺源性心脏病右室壁心肌运动定量分析研究[D]. 杭州: 浙江大学, 2007.
[2] Earley S, Heppner TJ, Nelson MT, et al. TRPV4 forms a novel Ca²⁺ signaling complex with ryanodine receptors and BKCa channels[J]. Circ Res, 2005, 97(12): 1270-1279.
[3] 陈慧勤. 慢性低氧及野百合碱对SD大鼠右心室功能及规范性瞬时感受器电位亚家族表达的影响[J]. 中国老年学杂志, 2015, 35(20): 5725-5727.
[4] 李淑琴, 朱嘉宝, 武宇洲. 银杏叶提取物防治心脑血管疾病的研究进展[J]. 中国新药杂志, 2016, 25(1): 76-81.
[5] Ohba T, Watanabe H, Murakami M, et al. Upregulation of TRPC1 in the development of cardiac hypertrophy[J]. Mol Cell Cardiol, 2007, 42(3): 498-507.
[6] Lin MJ, Leung GP, Zhang WM, et al. Chronic hypoxia-induced upregulation of store-operated and receptor-operated Ca²⁺ channels in pulmonary arterial smooth muscle cells: a novel mechanism of hypoxic pulmonary hypertension[J]. Circ Res, 2004, 95(5): 496-505.
[7] Eder P, Molkentin JD. TRPC channels as effectors of cardiac hypertrophy[J]. Circ Res, 2011, 108(2): 265-272.
[8] Kuwahara K, Wang Y, McAnally J, et al. TRPC6 fulfills a calcineurin signaling circuit during pathologic cardiac Remodeling[J]. Clin Invest, 2006, 116(12): 3114-3126.
[9] Onohara N, Mishida M, Inoue R, et al. TRPC3 and TRPC6 are essential for angiotensin II-induced cardiac hypertrophy[J]. EMBO, 2006, 25(22): 5305-5316.
[10]孙素红, 张宏生. 银杏达莫与丹参针剂对冠心病心绞痛治疗的临床效果观察[J]. 中药药理与临床, 2015, 31(1) : 230-232.
[11]李玥, 张群英, 罗礼云, 等. 银杏叶提取物对大鼠心肌梗死后转化生长因子-β1表达的影响[J]. 中华中医药学刊, 2016, 34(12): 2945-2947.
[12]陈琦, 詹亚梅, 谢娟, 等. 银杏叶提取物对去甲肾上腺素诱导的心肌细胞能量代谢障碍与凋亡的影响[J]. 现代医药卫生, 2016, 32(17): 2634-2636.

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