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CJAP ›› 2019, Vol. 35 ›› Issue (3): 245-249.doi: 10.12047/j.cjap.5775.2019.052

• ORIGINAL ARTICLES • Previous Articles     Next Articles

Effects of metformin on depressive behavior in chronic stress rats

LI Gai-fen1,2, ZHAO Ming2, ZHAO Tong2, CHENG Xiang2, FAN Ming1,2△, ZHU Ling-ling2△   

  1. 1. Center for Brain Disorders Research, Capital Medical University, Beijing Institute of Brain Disorders, Beijing 100069;
    2. Institute of Military Cognition and Brain Science, Academy of Military Medical Sciences, Beijing 100850, China
  • Online:2019-05-28 Published:2019-06-28

Abstract: Objective: To detect the effects of metformin on the depressive-like behaviors in rats. Methods: Forty male SD rats were randomly divided into four groups: control group (CON group), metformin group (MET group), model group (CUMS group), model + metformin group (CUMS + MET group), 10 rats in each group. Chronic unpredictable mild stress (CUMS) method was used to establish rat depression model in three weeks. After the model was established successfully, two metformin groups were intraperitoneally injected with metformin (100 mg/kg), while the control group and the model group were injected with the same amount of saline once a day for two weeks. After that, the changes of weight gain, sucrose water preference experiment, forced swimming test, tail suspension immobility test and open field test were detected. The morphological changes of hippocampus were observed by Nissl staining. Results: Compared with the control group, the weight gain of rats in CUMS group was significantly slowed down (P<0.05), the sucrose preference rate and the spontaneous activity were significantly reduced (P<0.05), and the immobility time in forced swimming and tail suspension immobility test was significantly prolonged (P<0.05), and the morphological structure of hippocampus was changed, which confirmed the success of CUMS depression model. Compared with CUMS group, metformin treatment had no significant effect on body weight of rats, but it could significantly improve sucrose water intake, immobility time and spontaneous activity of CUMS depression model rats (P<0.05), and improve the abnormal morphological changes of hippocampus in CUMS rats. Conclusion: Metformin has a therapeutic benefit against CUMS-induced depression, which provides a new treatment for patients with diabetes mellitus complicated with depression.

Key words: metformin, chronic unpredictable mild stress, rat, depressive behavior, hippocampus

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