Effects of Salvia mil on Th17 cells and related cytokines in non-alcoholic fatty liver disease

doi:10.12047/j.cjap.5846.2019.113

Abstract

Abstract: Objective: To study the effects of Salvia mil on Th17 cells and related cytokines in non-alcoholic fatty liver disease (NAFLD) rats. Methods: SD rats were randomly divided into control group, model group, Pueraria control group and Salvia mil treated group (8 rats in each group). Rats in Salvia mil treated group and Pueraria control group were treated with Salvia mil or Pueraria at the dose of 6.25g·kg^-1·d^-1 by gavage once a day for 4 weeks. Except for control group, rats in other groups were fed with high-fat diet for 4 weeks. The levels of serum lipids (TC, TG, LDL-C, HDL-C), liver function indexes (AST, ALT) and liver index were measured, and the pathological changes of liver tissues were observed. Th17 and Treg cells in peripheral blood were detected by flow cytometry. Serum levels of IL-6, IL-17 and TNF-alpha were detected by ELISA. The expression level of RORγt gene in liver tissue was detected by RT-PCR. Results: NAFLD rat model was successfully established by feeding high-fat diet for 4 weeks. The liver of model group had steatosis and a large number of inflammatory cells infiltrated. Compared with model group, the levels of TC, TG, LDL-C, ALT, AST and liver index in Salvia mil treated group and Pueraria control group were significantly lower than those of model group (P＜0.05), and the level of HDL-C was significantly higher than that of model group (P＜0.05). The content of Th17 cells in peripheral blood of rats in Salvia mil treated group was 1.58%±0.18%, significantly lower than that of model group 3.50%±0.16% and Pueraria control group 1.69%±0.20% (P＜0.05), Treg cell content was 4.58%±0.18%, higher than that of model group 3.71%±0.22% (P＜0.05) and Pueraria control group 4.52%±0.19%(P＞0.05), Treg/Th17 was 2.91±0.25, significantly higher than that of model group 1.06±0.24 and Pueraria control group 2.67±0.26 (P＜0.05). The levels of IL-6, IL-17 and TNF-α in Salvia mil treated group were lower than those of model group and Pueraria control group, and the levels of IL-6 and IL-17 were decreased significantly (P＜ 0.05). The expression level of RORγt gene in Salvia mil treated group was 0.72±0.09, significantly lower than that of model group 2.16±0.14 and Pueraria control group 1.32±0.08 (P＜0.05). Conclusion: Salvia mil can decrease the levels of IL-6, IL-17 and TNF-α, inhibit the expression of RORγt gene, decrease the content of Th17 cells, increase the content of Treg cells, adjust the balance of Th17/Treg, then inhibit the development of NAFLD.

Key words: Salvia mil, non-alcoholic fatty liver disease, Th17 cell, cytokines, immunomodulation, rat

CLC Number:

R318.06

CUI Gui-yu, BAI Jian, MIAO Lan-ying, LIN Da-yong, WANG Xue-liang, LIU Xi-cheng. Effects of Salvia mil on Th17 cells and related cytokines in non-alcoholic fatty liver disease[J]. CJAP, 2019, 35(6): 517-521.

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