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CJAP ›› 2020, Vol. 36 ›› Issue (2): 184-188.doi: 10.12047/j.cjap.5847.2020.041

• ORIGINAL ARTICLES • Previous Articles     Next Articles

Effects of α-enolase gene interference expression on proliferation and apoptosis of follicular granulosa cells from Zi geese

JI Hong1, SHAO Zi-yi1, XUE Lin-lin2, NIU Chun-yang1, ZHAN Xue-long1, YANG Chuang1, ZHEN Li1, YANG Huan-min1, LI Shi-ze1△   

  1. 1. College of Animal Science and Veterinary Medicine, HeilongjiangBayi Agricultural University, Daqing 163319;
    2. Heilongjiang Polytechnic, Shuangcheng 150111, China
  • Online:2020-03-28 Published:2020-07-31

Abstract: Objective: To study the effects of α-enolase (ENO1) gene interference expression on proliferation, and cell cycle of follicular granulosa cells from Zi geese. Methods: F1 follicular granulosa cells were primary cultured (mixed culture), which were divided into four groups: ENO1 interference expression group (RNAi), unrelated sequence group (NC), culture group (Control), transfection reagent group (Lip). The apoptosis rate and cell cycle phase of the interference group and the control group were detected by the flow cytometry. Results: ENO1 gene interference expression slowed the proliferation of granulosa cells, increased the apoptosis, and increased the proportion of granulosa cells in G2/M phase. Conclusion: ENO1 gene interference expression could cause G2/M phase arrest in primary cultured goose follicular granulosa cells, induce cell apoptosis and inhibit cell proliferation.

Key words: α-enolase, Zi goose, follicular granulosa, proliferation, apoptosis, cell cycle

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