The effects of prenatal radiation of mobile phones on white  matter in cerebellum of rat offspring

doi:10.12047/j.cjap.5880.2020.017

Abstract

Abstract: Objective: To evaluate the effects of prenatal radiation of 850～1 900 MHz mobile phone on white matter in cerebellum of adult rat offspring. Methods: Pregnant rats were randomly divided into short term maternal radiation group, long term maternal radiation group and control group. Rats in short term and long term maternal radiation group were exposed to 6 h/d and 24 h/d mobile phone radiation during 1-17 days of pregnancy, respectively. The cerebellums of offspring rats at the age of 3 month(n＝8)were taken. Cell morphology in cerebellum was studied by hematoxylin-eosin (HE) staining. The expressions of myelin basic protein (MBP), neurofilament-L (NF-L) and glial fibrillary acidic protein (GFAP) in cerebellum of rat offspring were detected by immunohistochemistry and Western blot.Results: Compared to control group, the morphological changes of purkinje cells in cerebellum were obvious in rat offspring of short term and long term maternal radiation group. Compared to control group, decreased MBP and NF-L expressions and increased GFAP expression were observed in long term maternal radiation group(all P＜0.05). Compared to short term radiation group, the expressions of MBP and NF-L were down-regulated (all P＜0.05) and the expression of GFAP was up- regulated(P＜0.05) in long term radiation group.Conclusion: Prenatal mobile phone radiation might lead to the damage of myelin and axon with activity of astrocytes in cerebellum of male rat offspring, which is related to the extent of radiation.

Key words: prenatal mobile phone radiation, offspring rats, myelin basic protein (MBP), neurofilament-L (NF-L), glial fibrillary acidic protein (GFAP)

YANG Mei-li, HONG Shan-yan, HUANG Hong-hong, LYU Guo-rong, WANG Ling-xing. The effects of prenatal radiation of mobile phones on white matter in cerebellum of rat offspring[J]. CJAP, 2020, 36(1): 77-81.

References 19

[1]	王凌星, 黄红红, 吕国荣. 产前850～1900 MHz手机暴露对子代大鼠齿状回PCNA和DCX表达的影响 [J]. 中国应用生理学杂志, 2018, 34(1): 35-42.
[2]	王凌星, 黄红红, 吕国荣. 妊娠期手机暴露对子代大鼠顶叶皮质星形胶质细胞的影响 [J]. 中华围产医学杂志, 2017, 20(11): 829-834.
[3]	Bihannic L, Ayrault O. Insights into cerebellar development and medulloblastoma [J]. Bull Cancer, 2016, 103(1): 30-40.
[4]	Rahimi-Balaei M, Afsharinezhad P, Bailey K, et al. Embryonic stages in cerebellar afferent development [J]. Cerebellum Ataxias, 2015, 2: 7.
[5]	Odaci E, Hanci H, Ikinci A, et al. Maternal exposure to a continuous 900 MHz electromagnetic field provokes neuronal loss and pathological changes in cerebellum of 32-day-old female rat offspring [J]. J Chem Neuroanat, 2016, 75(Pt B): 105-110.
[6]	Haghani M, Shabani M, Moazzami K. Maternal mobile phone exposure adversely affects the electrophysiological properties of Purkinje neurons in rat offspring [J].Neuroscience, 2013, 250: 588-598.
[7]	Aldad TS, Gan G, Gao XB, et al. Fetal radiofrequency radiation exposure from 800～1900 MHZ-rated cellular telephones affects neurodevelopment and behavior in mice [J]. Sci Rep, 2012, 2(6): 312-319.
[8]	Goldstein JM, Handa RJ, Tobet SA. Disruption of fetal hormonal programming (prenatal stress) implicates shared risk for sex differences in depression and cardiovascular disease [J].Front Neuroendocrinol, 2014, 35(1): 140-158.
[9]	Li HP, Miki T, Gu H, et al. The effect of the timing of prenatal X-irradiation on Purkinje cell numbers in rat cerebellum [J]. Brain Res Dev Brain Res, 2002, 139(2): 159-166.
[10]	Jahn O, Tenzer S, Werner HB. Myelin proteomics: molecular anatomy of an insulating sheath [J]. Mol Neurobiol, 2009, 40(1): 55-72.
[11]	Johansson O, Redmayne M. Exacerbation of demyelinating syndrome after exposure to wireless modem with public hotspot [J]. Electromagn Biol Med, 2016, 35(4): 393-397.
[12]	Bahreyni Toossi MH, Sadeghnia HR, Mohammad Mahdizadeh Feyzabadi M, et al. Exposure to mobile phone (900～1800 MHz) during pregnancy: tissue oxidative stress after childbirth [J]. J Matern Fetal Neonatal Med, 2018, 31(10): 1298-1303.
[13]	Ikinci A, Mercantepe T, Unal D, et al. Morphological and antioxidant impairments in the spinal cord of male offspring rats following exposure to a continuous 900 MHz electromagnetic field during early and mid-adolescence [J]. J Chem Neuroanat, 2016, 75(Pt B): 99-104.
[14]	Kang TC, Park SK, Hwang IK, et al. The decreases in calcium binding proteins and neurofilament immunoreactivities in the Purkinje cell of the seizure sensitive gerbils [J]. Neurochem Int, 2002, 40(2): 115-122.
[15]	Powell EM, Geller HM. Dissection of astrocyte-mediated cues in neuronal guidance and process extension [J]. Glia, 1999, 26(1): 73-83.
[16]	Lutz SE, Zhao Y, Gulinello M, et al. Deletion of astrocyte connexins 43 and 30 leads to a dysmyelinating phenotype and hippocampal CA1 vacuolation [J]. J Neurosci, 2009, 29(24): 7743-7752.
[17]	Seifert G, Schilling K, Steinhauser C. Astrocyte dysfunction in neurological disorders: a molecular perspective [J]. Nat Rev Neurosci, 2006, 7(3): 194-206.
[18]	Allaman I, Belanger M, Magistretti PJ. Astrocyte-neuron metabolic relationships: for better and for worse [J]. Trends Neurosci, 2011, 34(2): 76-87.
[19]	何滔, 杨术旺, 郭赟, 等. 体外培养大鼠星形胶质细胞液压冲击损伤的研究[J]. 中国应用生理学杂志, 2010, 26(1): 48-52.

The effects of prenatal radiation of mobile phones on white matter in cerebellum of rat offspring

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[1]	WANG Ling-xing, HUANG Hong-hong, LV Guo-rong. cts of prenatal exposure of 850-1900MHz mobile phone on the expression of PCNA and DCX in dentate gyrus of offspring rats [J]. CJAP, 2018, 34(1): 35-38.
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