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CJAP ›› 2020, Vol. 36 ›› Issue (5): 390-393.doi: 10.12047/j.cjap.5896.2020.083

• ORIGINAL ARTICLES • Previous Articles     Next Articles

Effects of Apelin-13 on barrier injury of human umbilical vein endothelial cells induced by LPS

LIN Dao-peng1, CHEN Sha2, LU Li-ling1, WANG Yu3, LYU Fang-fang1, SHI Lin-wei1, KONG Xiao-xia3, SHAN Xiao-ou1, ZHANG Hai-lin1△   

  1. 1. The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou 325035;
    2. Department of Clinical Laboratory, Huangshi Central Hospital of Erdong Medical Group Affiliated Hospital of Hubei Polytechnic University, Huangshi 435000;
    3. School of Basic Medicine, Institute of Hypoxia Research, Wenzhou Medical University, Wenzhou 325035, China
  • Received:2019-06-19 Revised:2020-06-24 Published:2021-02-25

Abstract: Objective: To investigate the effects of Apelin-13 on barrier function injury of human umbilical vein endothelial cells (HUVECs) induced by LPS. Methods: The HUVECs cultured in vitro were divided into 4 groups: Control group, LPS group, Apelin-13+LPS group, Apelin-13 group. HUVECs were treated by 5 μg/ml LPS for 24 h to replicate the model with endothelial barrier impaired. Apelin-13 at the concentration of 1 μmol/L was given 30 min before LPS treatment. The cell viabillity of HUVECs was measured by CCK-8 assay. Protein expressions of VE-cadherin and F-actin were measured by Western blot and immunofluorescence. Nuclear factor κB p65(NF-κB p65) was detected by immunofluorescence. Results: Compared with the control group, the cell viabillity of HUVECs and protein expression of VE-cadherin were decreased by LPS, but the protein expression of F-actin and activation of NF-κB p65 were increased by LPS. These effects were attenuated by Apelin-13 administration. Conclusion: Apelin-13 ameliorates LPS-induced barrier function injury of HUVECs, which may be related to the inhibition of inflammation.

Key words: endothelial barrier, Apelin-13, HUVECs, adherens junctions, inflammation

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