Effect  of psoralen on rat osteoblasts injuries induced by  TCP wear particles in vitro and its mechanism

doi:10.12047/j.cjap.5900.2020.056

Abstract

Abstract: Objective: To investigate the effect and mechanism of psoralen on calvarial osteoblasts injuries caused by tricalcium phosphate (TCP) wear particles in vitro.Methods: Primary osteoblasts were obtained from the calvaria of neonatal SD rat by the series of digestion and were identified with ALP staining. Calvarial osteoblasts were treated with TCP wear particles for 48 h to establish the in vitro model of osteoblasts injuries. The rat osteoblasts were randomly divided into control group, TCP wear particles (0.1 mg/ml) group, psoralen treated (at the concentrations of 10^-7, 10^-6, 10^-5 mol/L) groups. WST assay and the flow cytometry were used to detect the cell viability of osteoblasts and apoptosis, respectively. Chemical colorimetry was performed to examine ALP activity of osteobalsts. When the osteoblasts were treated for 14 day, mineral nodules formation was observed with alizarin red S staining. Western blot was applied to examine protein expressions of glucose regulated protein78/94(GRP78/94), inositol dependent enzyme 1 alpha (IREα), spliced X-box binding protein 1 (XBP1s) and phosphorylated c-Jun N-terminal kinase (p-JNK) in calvarial osteoblasts. Results: Compared with control group, the cell viability of osteoblasts, ALP activity and mineral nodules formation in TCP group were decreased significantly (P＜0.05), while the percentage of apoptosis and protein expressions of GRP78/94, IRE1α, XBP1 and p-JNK were obviously increased in calvarial osteoblasts (P＜0.05). Compared with TCP group, the injuries of calvarial osteoblasts and cell apoptosis in psoralen treated groups were obviously decreased (P＜0.05), and the expression levels of GRP78/94, IRE1α, XBP1 and p-JNK were down-regulated remarkably (P＜0.05). Conclusion: Psoralen prevents osteoblasts injuries caused by TCP wear particles through IRE1α-XBP1s-JNK signaling pathway activation.

Key words: TCP wear particles, osteoblasts, psoralen, apoptosis, ER stress, rat

CLC Number:

R363.3

CHEN Yu-feng, DONG Fan-he, LOU Yun-wei, SHOU Jin-hao, ZHANG Hui-ting, ZHOU Yi-chao, YAN Ming, MAO Hong-jiao, ZHANG Yun. Effect of psoralen on rat osteoblasts injuries induced by TCP wear particles in vitro and its mechanism[J]. CJAP, 2020, 36(3): 255-260.

References

[1] Lochner K, Fritsche A, Jonitz A, et al. The potential role of human osteoblasts for periprosthetic osteolysis following exposure to wear particles [J]. Int J Mol Med, 2011, 28: 1055-1063.
[2] Vermes C, Roebuck KA,Chandrasekaran R, et al. Particulate wear debris activates protein tyrosine kinases and nuclear factor kappa Beta, which down-regulates type I collagen synthesis in human osteoblasts [J]. J Bone Miner Res, 2000, 15: 1756-1765.
[3] Wang Z, Liu N, Liu K, et al. Autophagy mediated CoCrMo particle-induced peri-implant osteolysis by promoting osteoblast apoptosis [J]. Autophagy, 2015, 11(12): 2358-2369.
[4] Zhu Z, Xie Q, Huang Y, et al. Aucubin suppresses Titanium particlesmediated apoptosis of MC3T3E1 cells and facilitates osteogenesis by affecting the BMP2/Smads/RunX2 signaling pathway [J]. Mol Med Rep, 2018, 18(3): 2561-2570.
[5] Klinder A, Seyfarth A, Hansmann D, et al. Inflammatory response of human peripheral blood mononuclear cells and osteoblasts incubated with metallic and ceramic submicron particles [J]. Front Immuno, 2018, 9: 831.
[6] 刘国印, 王瑞, 董磊, 等. 内质网应激介导成骨细胞凋亡在骨溶解骨组织中的作用及机制[J]. 中国组织工程研究, 2014, 18(33): 5257-5265.
[7] Wang X, Xu C, Hua Y, et al. Psoralen induced cell cycle arrest by modulating Wnt/β-catenin pathway in breast cancer cells [J]. Sci Rep, 2018, 8(1): 14001-14001.
[8] 高海南, 刘斌, 刘国良, 等. 补骨脂素对Aβ损伤PC12细胞的保护作用研究[J]. 中药材, 2019, 42(1): 175-178.
[9] Tang DZ, Yang F, Yang Z, et al. Psoralen stimulates osteoblast differentiation through activation of BMP signaling [J]. Biochem Biophys Res Commun, 2011, 405(2): 256-261.
[10]Chen S, Wang Y, Yang Y, et al. Psoralen inhibited apoptosis of osteoporotic osteoblasts by modulating IRE1-ASK1-JNK pathway [J]. Biomed Res Int, 2017: 3524307.
[11]张月红, 高兰兴, 金宏, 等. 染料木黄酮对成骨细胞增殖分化的影响[J]. 中国应用生理学杂志, 2003, 19(2): 182-184.
[12]孔令宇, 席錾, 马文婷, 等. 缺氧缺糖诱导心肌细胞损伤中Notch信号对HIF-α及自噬相关基因Beclin1, LC3Ⅰ 和LC3Ⅱ表达的影响[J]. 中国应用生理学杂志, 2019, 35(2): 165-168.
[13]Gallo J, Goodman SB, Konttinen YT, et al. Particle disease: biologic mechanisms of periprosthetic osteolysis in total hip arthroplasty [J]. Innate Immun, 2013, 19: 213-224.
[14]Deng Z, Wang Z, Jin J, et al. SIRT1 protects osteoblasts against particle-induced inflammatory responses and apoptosis in aseptic prosthesis loosening [J]. Acta Biomater, 2016, 49: 541-554.
[15]林坤, 陈佳濠, 张云, 等. 原花青素对磷酸三钙磨损颗粒所致小鼠颅骨溶解的干预作用及其机制[J]. 中国应用生理学杂志, 2019, 35(2): 172-177.
[16]Sato AY,Tu X, McAndrews KA, et al. Prevention of glucocorticoid induced-apoptosis of osteoblasts and osteocytes by protecting against endoplasmic reticulum (ER) stress in vitro and in vivo in female mice [J]. Bone, 2014, 73: 60-68.
[17]Liu W, Xu C, Ran D, et al. CaMKⅡ mediates cadmium induced apoptosis in rat primary osteoblasts through MAPK activation and endoplasmic reticulum stress [J]. Toxicology, 2018, 407(1): 70-80.
[18]Yan TB, Li C, Jiao GJ, et al. TIMP-1 suppressed by miR-138 participates in endoplasmic reticulum stress-induced osteoblast apoptosis in osteoporosis [J]. Free Radic Res, 2018, 52(2): 223-231.
[19]Yang F, Tang J, Dai K, et al. Metallic wear debris collected from patients induces apoptosis in rat primary osteoblasts via reactive oxygen speciesmediated mitochondrial dysfunction and endoplasmic reticulum stress [J]. Mol Med Rep, 2019, 19(3): 1629-1637.
[20]薛欣, 赵京元, 张永祥. 肿瘤坏死因子α诱导ATF6、IRE1α介导的未折叠蛋白反应 [J]. 毒理学杂志, 2008, 22(6): 431-434.
[21]Jiang C, Zhang S, Liu H, et al. The role of the IRE1 pathway in PBDE-47-induced toxicity in human neuroblastoma SH-SY5Y cells in vitro [J]. Toxicol Lett, 2012, 211(3): 325-333.
[22]Yao W, Yang X, Zhu J, et al. IRE1α siRNA relieves endoplasmic reticulum stress-induced apoptosis and alleviates diabetic peripheral neuropathy in vivo and in vitro [J]. Sci Rep, 2018, 8(1): 2579-2579.

Effect of psoralen on rat osteoblasts injuries induced by TCP wear particles in vitro and its mechanism

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