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CJAP ›› 2020, Vol. 36 ›› Issue (3): 235-239.doi: 10.12047/j.cjap.5953.2020.052

• Original Articles • Previous Articles     Next Articles

Effects of NaHS on MBP and learning and memory in hippocampus of mice with spinocerebellar ataxia

JIANG Hong-bo1, DONG Jia-xing2, QIN Yu-fei2, LIU Jia-cong2, JIANG Wan-ju2, LI Ruo-nan2, LIU Lan-ci2, TIAN Yi-dan2, XU Yu-ming, DU Ai-lin1,2Δ   

  1. 1. The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang 453000;
    2. Department of Physiology and Neurobiology of Xinxiang Medical University, Joint Laboratory of Brain Functional Injury between China and Britain, Fostering Base of Key Laboratory of Brain Research in Henan Universities, Xinxiang 453000;
    3. The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
  • Received:2019-10-16 Revised:2020-04-17 Published:2020-09-25

Abstract: Objective: To investigate the effects of exogenous NaHS on myelin basic protein (MBP) and learning and memory of hippocampal neurons in mice with spinocerebellar ataxia type 3 (SCA3) and its therapeutic significance.Methods: Twelve male normal mice were randomly selected as normal control group (NC Group), and 48 SCA3 mice were randomly selected as SCA3 model group (M Group), low dose group (NL Group, 10 μmol/kg), medium dose group (NM Group, 50μmol/kg) and high dose group (NH Group, 100 μmol/kg), 12 rats in each group. The drug treated groups were injected with NaHS intraperitoneally once a day for 4 weeks. The changes of learning and memory ability of SCA3 mice before and after the intervention of different doses of NaHS were determined by Morris water maze, the content of hydrogen sulfide (H2S) in hippocampus was measured by spectrophotometry, the expression of MBP was detected by immunohistochemistry, and the morphological changes of neuron myelin sheath were observed by electron microscope. Results: Compared with the control group, the learning and memory ability of SCA3 mice was decreased significantly (P<0.05), and the content of H2S in hippocampus was decreased (P<0.05). After different doses of exogenous NaHS treatment, the learning and memory ability was improved in different degrees (P<0.05), and the contents of H2S and MBP in hippocampus of SCA3 mice were also improved in different degrees (P<0.05). Conclusion: Exogenous NaHS may increase the contents of H2S and MBP in the hippocampus of SCA3 mice, which may have a protective effect on the neurons, and then improve the learning and memory ability of SCA3 mice, and provide a new idea for the treatment of SCA3.

Key words: NaHS, learning and memory, SCA3 mice, H2S, MBP, hippocampus

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