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CJAP ›› 2020, Vol. 36 ›› Issue (4): 318-320.doi: 10.12047/j.cjap.5965.2020.068

• ORIGINAL ARTICLES • Previous Articles     Next Articles

Effects of exogenous H2S on hepatic fibrosis in diabetic mice and its mechanism

XI Yu-xin, WEN Xin, JIAO Li-jie, WEI Ya-xin, CHANG Gui-quan, WU Ren, SUN Feng-qi, HAO Jing-hui, LI Hong-zhu   

  1. Departmentof Pathophysiology, Harbin Medical University, Harbin 150086, China
  • Received:2019-11-01 Revised:2020-06-16 Published:2020-11-09

Abstract: Objective: To investigate the effects of exogenous hydrogen sulfide (H2S) on the hepatic fibrosis in diabetic mice and its mechanism. Methods: Twenty-four C57 male mice (weight 22±2 g) were randomly divided into three groups (n=8): ① Normal control group (Control): Mice were intraperitoneally injected equal amount of normal saline, the injection time was the same as that of the experimental groups; ② Diabetes model groups (HG): Streptozotocin (STZ) was injected intraperitoneally once according to body weight (150 mg/kg) to establish diabetes model; ③ NaHS treatment groups (HG + NaHS): Mice were intraperitoneally injected with NaHS (100 μmol/L·kg·d) once a day for 12 consecutive weeks. The hepatocyte injury was detected by HE staining; the hepatic fibrosis was observed through Masson staining; the protein expressions of cystathionine - β - synthetase (CBS), collagen-I (CoL-I), collagen-III (CoL-III) and matrix metalloproteinase-9 (MMP-9) were detected by Western blot. Results: Compared with the control group, the damage and fibrosis of hepatocyte were significantly aggravated, the expression of CBS proteins was decreased (P<0.01), and the expression levels of CoL-I, CoL-III and MMP-9 proteins were increased (P<0.01) in the diabetic model group. Compared with the diabetic model group, the damage and fibrosis of hepatocyte were significantly lightened, the expression of CBS proteins was obviously increased (P<0.01), and the expression levels of CoL-I, CoL-III and MMP-9 proteins were markedly decreased (P< 0.01). Conclusion: H2S inhibits the hepatic fibrosis in diabetic mice, and its mechanism is related to the decrease of collagen and matrix metalloproteinase-9.

Key words: hydrogen sulfide, hepatic fibrosis, diabetes, mice

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