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CJAP ›› 2020, Vol. 36 ›› Issue (5): 438-443.doi: 10.12047/j.cjap.5991.2020.093

• ORIGINAL ARTICLES • Previous Articles     Next Articles

Effects of FABP5-PPARγ signaling pathway on learning-memory ability and lipid metabolism in rats with vascular dementia

CHEN Dan-dan1, DONG Ying2, YANG Xiao-dan3△   

  1. 1. Department of Cerebrovascular Diseases, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300300;
    2. Department of Radiation Ⅲ, Tumor Hospital of Jilin Province, Changchun 130012;
    3. Department of Neurology, the 983th Hospital of People's Liberation Army, Tianjin 300142, China
  • Received:2019-12-19 Revised:2020-06-09 Published:2021-02-25

Abstract: Objective: To investigate the effects of fatty acid binding protein 5(FABP5)- peroxisome proliferator-activated receptor gamma(PPARγ) signaling pathway on learning-memory ability and lipid metabolism in rats with vascular dementia(VD) and its mechanisms. Methods: ①VD model rats were established by ligating bilateral common carotid artery. These rats were divided into three groups: normal group (WT group), sham-operated group (sham group) and VD model group; ②WT group and FABP5 inhibitor group were set up. After four weeks, Morris water maze test was used to detect spatial learning and memory ability in rats. RT-qPCR and Western blot methods were used to detect the expressions of FABP5, PPARγ, p-PPARγ and lipoprotein lipase (LPL) in the brain at the mRNA and protein levels. The levels of TC, TG and FFA in the brain were detected by assay kits. Results: Compared with the WT group and sham group, the learning-memory ability of the VD model and the FABP5 inhibitor group were significantly decreased, and the expressions of FABP5, PPARγ, p-PPARγ and LPL were significantly decreased at mRNA level and protein level in the brain; and the levels of TC, TG and FFA were increased significantly in the brain. Conclusion: FABP5 can affect the learning-memory ability and lipid metabolism in VD rats through PPARγ and LPL.

Key words: Vascular dementia, fatty acid binding protein 5, peroxisome proliferator-activated receptor gamma, lipoprotein lipase, lipid metabolism

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