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CJAP ›› 2020, Vol. 36 ›› Issue (6): 622-627.doi: 10.12047/j.cjap.5993.2020.130

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Effects of LncRNA UNC5B-AS1 on adhesion, invasion and migration of lung cancer cells and its mechanism

TAN Jian-Jun1, LONG Shu-Zi2, ZHANG Tao2△   

  1. 1. Department of Oncology, Chongqing Three Gorges Central Hospital, Chongqing 404000;
    2. Department of Oncology, First Affiliated Hospital of Chongqing Medical University of Chongqing, Chongqing 404000, China
  • Received:2019-12-23 Revised:2020-09-09 Online:2020-11-28 Published:2021-03-15

Abstract: Objective: To investigate the effects of long-chain non-coding RNA (lncRNA) UNC5B-AS1 on the adhesion, invasion and migration of lung cancer cells by regulating the expression of miR-218-5p. Methods: Twenty specimens of lung cancer patients and corresponding paracancerous tissues were surgically removed and collected from the oncology department of Chongqing Three Gorges Central Hospital from June 2017 to June 2019. Real-time quantitative PCR (qRT-PCR) was used to detect the expressions of UNC5B-AS1 in human bronchial epithelial cells HBE and different lung cancer cells of A549, H1437, H1975, H1299 and H460. UNC5B-AS1 siRNA was transfected into lung cancer A549 cells. Adhesion assay, transwell invasion assay and scratch assay were used to detect the effect of UNC5B-AS1 on adhesion, invasion and migration of A549 cells. qRT-PCR and dual luciferase reporter gene were used for the detection and identification of UNC5B-AS1 targeting miR-218-5p. The expression of epithelial-mesenchymal transition (EMT)-related protein was detected by Western blot. Results: The expression of UNC5B-AS1 in lung cancer tissues and cells was significantly higher than that in adjacent tissues and bronchial epithelial cells (P<0.05). The expression of UNC5B-AS1 in lung cancer A549 cells was the highest (P<0.05). Down-regulation of UNC5B-AS1 expression inhibited adhesion, invasion and migration of A549 cells (P<0.05). qRT-PCR and dual luciferase reporter assay experiments showed that UNC5B-AS1 targeted the regulation of miR-218-5p expression. Down-regulation of UNC5B-AS1 inhibited E-cadherin protein expression and promoted Vimentin and Twist protein expression. Conclusion: lncRNA UNC5B-AS1 promotes adhesion, invasion and migration of lung cancer cells through targeted regulation of miR-218-5p expression, and its mechanism may be related to the promotion of EMT.

Key words: UNC5B-AS1, miR-218-5p, lung cancer cells, adhesion, invasion, migration

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