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CJAP ›› 2021, Vol. 37 ›› Issue (4): 433-438.doi: 10.12047/j.cjap.6064.2021.034

• ORIGINAL ARTICLES • Previous Articles     Next Articles

Effects of astaxanthin combined with aerobic exercise on renal aging of rat induced by D-galactose and its mechanism

NIU Yan-long1,2, CAO Jian-min3, ZHOU Qi-yun4,5, HU Ge6, GUO Xian3, LIU Jian4, HAO Min4, ZHANG Tao3, ZHOU Hai-tao4,5△   

  1. 1. Gannan Medical University, Jiangxi Ganzhou 341000, China;
    2. Key Laboratory of Rehabilitation Medicine in Ganzhou, Jiangxi Ganzhou 341000, China;
    3. Beijing Sport University, Beijing 100084;
    4. Beijing Union University, Beijing 100101;
    5. Key Laboratory of Bioactive Substances and Functional Foods, Beijing Union University, Beijing 100191;
    6. Changzhou University, Changzhou 213164, China
  • Online:2021-07-28 Published:2021-08-09

Abstract: Objective: To study the effects and mechanisms of astaxanthin combined with aerobic exercise on renal senescence of rat induced by D-galactose. Methods: Sixty 3-month-old SPF SD rats were divided into control group (C group), acute senescence group (S group), astaxanthin+acute senescence group (AS group), aerobic exercise+acute senescence group (ES group), astaxanthin+aerobic exercise+acute senescence group (AES group), by two-factor two-level 2×2 factorial design with 12 rats in each group. Acute senescence model of rat was establshed by intraperitoneal injection with 100 mg/(kg·d) D-galactose, and the intervention was conducted with 20 mg/(kg·d) astaxanthin and/or aerobic exercise with 60% VO2max for 6 weeks. The histopathological/ultrastructural changes of the kidney were observed by light microscope/electron microscope; the levels of SOD, γ-GCS and MDA were detected by ELISA, and LDF in kidney was determined by fluorescence colorimetry; the protein expression of Nrf2 signaling pathway was detected by immunohistochemistry. Results: Compared with AS and ES group, in AES group, the improvement of renal tissue morphology/ultrastructure was more significant; LDF was decreased significantly (P<0.01); SOD activity was significantly increased (P<0.01); γ-GCS was significantly higher than that of AS group, but not significantly different from that of ES group (P>0.05); there was no significant difference in MDA between groups (P>0.05); the levels of Nrf2 and p-Nrf2 were increased significantly (P<0.05, P<0.01); HO-1 was significantly higher than that of ES group(P<0.05), but not significantly different compared with that of AS group(P>0.05). Conclusion: Astaxanthin combined with aerobic exercise can delay aging process of kidney, its mechanism may be that the combination regulate the protein expression in Nrf2 signaling pathway, Ⅱ detoxifying enzymes and antioxidant enzyme activity, and improve oxidative stress in kidney of rat induced by D-galactose.

Key words: astaxanthin, aerobic exercise, aging, oxidative stress, Nrf2, kidney

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