The effects of aerobic exercise plus spirulina polysaccharide supplement on learning and memory function of diabetes rats and its mechanism

doi:10.12047/j.cjap.6065.2021.058

Abstract

Abstract: Objective: To investigate the effects of aerobic exercise plus spirulina polysaccharide(SP) supplement on the related protein expressions of p75^NTRsignal in hippocampal and the improvement of learning and memory of type 2 diabetes rats. Methods: The model of type 2 diabetic rats was established by fed high-fat diet for four weeks together with intraperitoneal injecting a low dose of STZ. The model rats were randomly divided into diabetic model group(B),diabetic exercise group(C),diabetic+SP group(D)and diabetic exercise+SP group(E), another normal control group(A)without any intervention was set up,12 rats in each group. The rats in Group C and E were treated with intervention of swimming training for six weeks. The rats in Group D and E were treated with SP intragastrically for 6 weeks. Learning and memory abilities were observed by Morris water maze test. The hippocampus cell apoptosis was observed by Tunnel staining, and BDNF content and the expressions of p75^NTR, cleaved caspase-3 of hippocampus were tested by ELISA, Western blot and immunohistochemistry, respectively. At the same time, the changes of blood glucose and levels of serum insulin were examined. Results: ①Compared with Group A at different time points, the body weight of Group B was decreased significantly(P＜0.01). Compared with Group B at different time points, the body weight of Group C,D and E had no difference (P＞0.05). Compared with Group A, levels of the blood glucose and serum insulin Group B were increased significantly(P＜0.01).Compared with Group B, the levels in the intervention groups were decreased significantly (P＜0.01); ②Compared with Group A, the escape latencies of Group B were prolonged significantly(P＜0.01), platform quadrant residence duration and the times of crossing platform were decreased (P＜0.01). Compared with Group B, the escape latencies of the intervention groups were shortened (P＜0.05 or P＜0.01), and the times of crossing platform were increased (P＜0.05 or P＜0.01). ③Compared with Group B, the neural cells apoptosis of the intervention rats was decreased, and the protein expressions of p75^NTR and cleaved caspase-3 were decreased (P＜0.05 or P＜0.01), however the expression of BDNF was increased significantly (P＜0.05 or P＜0.01). Conclusion: Aerobic exercise and SP supplement can improve the learning-memory ability of type 2 diabetes rats, and the improvement effect of exercise combined with SP is markedly better than that of exercise and SP alone, the mechanism might be related to better regulating p75^NTR signal related protein expressions and then inhibiting apoptosis in hippocampus of rats with type 2 diabetes.

Key words: aerobic exercise, spirulina polysaccharide, p75^NTR signal, cell apoptosis, learning and memory

CLC Number:

G804
R49

ZHU Hong-zhu, ZHU Mei-ju, ZHANG Ying. The effects of aerobic exercise plus spirulina polysaccharide supplement on learning and memory function of diabetes rats and its mechanism[J]. CJAP, 2021, 37(6): 665-672.

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