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CJAP ›› 2020, Vol. 36 ›› Issue (6): 605-610.doi: 10.12047/j.cjap.6067.2020.127

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Effects of Bushen Zhuanggu Granules on expressions of serum GH with IGF-1 and its receptors in bone tissue of ovariectomized rats

SU Hai-rong1,4, CHENG Wen-yao2, YUAN Qiu-hong1, OUYONG Jing1,4, DENG Wei-min1,3△   

  1. 1. General Hospital of Southern Theatre Command, Department of Rehabilitation;
    2. General Hosp -ital of Southern Theatre Command, Department of military Patient Management, Guangzhou 510010;
    3. Qianhai life insurance Guangzhou General Hospital, Department of Rehabilitation, Guangzhou 511300;
    4. Guangzhou University of Chinese Medicine, Guangzhou 510405, China
  • Received:2020-03-30 Revised:2020-11-20 Online:2020-11-28 Published:2021-03-15

Abstract: Objective: To investigate the effects of Bushen Zhuanggu granule on the expressions of serum growth hormone (GH) and insulin-like growth factor-1(IGF-1) and their receptors in bone tissues of ovariectomized rats. Methods: Forty-eight SD female rats (weight 273±21.3 g) were divided into 4 four groups: the dosage of Bushen Zhuanggu granule group (BSZG) was 2.5 g/(kg·d),the do -sage of estradiol group(E2) was 0.071mg/(kg·d),sham group (SHAM) and ovariectomized model group (OVX group) were given the same amount of saline by oral administration.Each group included 12 rats,the treatments were conducted once a day. After 3 and 6 months of treatment,bone mineral density (B -MD) was measured by bone density instrument;the serum levels of GH and IGF-1 were detected by EL-ISA;the expressions of GHR and IGF-1R of bone tissue were detected by qPCR;the optical density(OD)value and positive cell count of pituitary GH immunohistochemical tablets were analyzed by Image J software,respectively. Results: ①After 3 months intervention,compared with the SHAM, the BMD of the spine in E2 group was increased(P<0.05),and the BMD of both parts in BSZG group were increased(P<0.05).After two stage intervention,BMD of both part in the two drug groups was higher than that in the OVX group(P<0.05).②After two-stage intervention,the expression levels of serum GH and IGF-1,GHR and IGF-1R in BSZG group were higher than those in OVX group (P<0.05).The levels of serum GH and it's receptors in the E2 group were increased (P<0.05), but the serum IGF-1 level remained unchanged (P>0.05) or even was decreased (P<0.05).③After two stage in -tervention,the OD values and the positive cells count in the two drug intervention groups were increased (P>0.05).④Pearson correlation analysis showed that serum GH,IGF-1 concentration and it's receptors in bone tissue were positively related with BMD. Serum GH concentration was positively correlated with OD values and number of positive cells. Conclusion: Bushen Zhuanggu granule can be used to improve the expressions of GH,IGF-1 in serum and its receptors in bone tissues of ovariectomized osteoporosis rats to prevent further loss of bone mass and increase bone mineral density.

Key words: Bushen Zhuanggu granule, postmenopausal osteoporosis, growth hormone, insulin-like growth factor-1, bone mineral density, estradiol

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