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CJAP ›› 2021, Vol. 37 ›› Issue (4): 419-422.doi: 10.12047/j.cjap.6078.2021.039

• ORIGINAL ARTICLES • Previous Articles     Next Articles

Effects of betulinic acid on apoptosis of human gastric cancer MGC-803 cells

FAN Hua1, SHAO Shu-li 1,2Δ, HE Meng-qi1, HUANG Xin 1,2, ZHANG Wei-wei1,2, ZHANG Zhen-zhu1,2   

  1. 1. College of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihaer 161000;
    2. Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar University, Qiqihaer 161000, China
  • Online:2021-07-28 Published:2021-08-09

Abstract: Objective: The effects of betulinic acid (BA) on apoptosis of human gastric cancer MGC-803 cells was investigated by using human gastric cancer MGC-803 cells as experimental materials, and the basis for its clinical application was provided. Methods: The human gastric cancer MGC-803 cells were divided into 4 groups,each group was set with 3 replicates.The control group was MGC-803 cells without being added betulinic acid; the other 3 groups of experimental groups were treated with betulinic acid at final concentrations of 10, 20 and 30 μg /ml respectively. Cells were treated with betulinic acid of different concentrations for 48 h. Laser confocal microscope was used to observe morphological changes of MGC-803. The activities of Caspase-3 and Caspase-9 were detected by an assay kit. Flow cytometry was applied to determine mitochondrial membrane potential. The mRNA and protein levels of Caspase-3, Caspase-9 and Cyt c were also detected by qRT-PCR and Western blot, respectively. Results: Compared with the control group, the activities of Caspase-3 and caspase-9 were increased(P<0.01), while the mitochondrial membrane potential was decreased significantly(P<0.01). The mRNA and protein expressions of Caspase-3, caspase-9 and Cyt c were up-regulated significantly(P<0.01). Conclusion: In the final concentration range of 10 ~ 30 μg/ml, betulinic acid can induce apoptosis of human gastric cancer MGC-803 cells by regulating the expression of Caspase-3, Caspase-9 and Cyt c.

Key words: betulinic acid, human gastric cancer MGC-803, cell apoptosis

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