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CJAP ›› 2021, Vol. 37 ›› Issue (6): 673-677.doi: 10.12047/j.cjap.6081.2021.091

• ORIGINAL ARTICLES • Previous Articles     Next Articles

Effects of long-term moderate-small intensity aerobic exercise on differential expression of proteome in left ventricular muscle of rats

GUO Yuan-pan1, SHI Shao-rong2△, XU Zhe3, WANG Juan4, HUANG Si-min5, WAN Li-li6, LEI Xiong6, PENG Yong7   

  1. 1. P. E. Dept.,Changsha Normal University, Changsha 410010;
    2. P. E. Dept., Hunan Normal University, Changsha 410012;
    3. P. E. Dept., Hunan City University, Yiyang 413039;
    4. Beijing Sport University, Beijing 100084;
    5. Sports and Health Department, Zhaoqing University, Zhaoqing 526061;
    6. Xiangya Hospital, Central South University, Changsha 410008;
    7. Nanjing Sport Institute, Nanjing 210014, China
  • Received:2020-04-24 Revised:2021-03-01 Online:2021-11-28 Published:2021-11-25

Abstract: Objective: To investigate the effects of long-term moderate-small intensity aerobic exercise on the differential expression of proteome in left ventricular muscle of rats, and to screen the target proteins sensitive to moderate-small intensity aerobic exercise stimulation. This study will enrich the basic theory of exercise and fitness and provide new ideas and experimental basis for the rehabilitation treatment of chronic cardiovascular disease. Methods: Twenty male SD rats were randomly divided into exercise group and control group (n=10). The treadmill training model of long-term moderate-small intensity aerobic exercise was established, and the whole protein samples of left ventricular muscle were extracted and separated by two-dimensional gel electrophoresis (2-DE). The two-dimensional gel electrophoresis map was analyzed by Bio PD quest image analysis software. The protein spots with differential expression more than 5 times or down-regulated over 80% after exercise were identified by tandem time-of-flight mass spectrometry (ULGRAFL-FLEX-TOF/TOF). Results: Compared with the group C, the heart weight index of the group E was increased by 32.0%, and the difference was significant (P<0.05). Compared with the group C, there were 71 protein spots expression were up-regulated≥2 times or down-regulated≥50% in the group E. 4 protein spots expression were up-regulated≥5 times or down-regulated≥80% were identified by mass spectrometry, 3 proteins and 1 unknown protein were identified. Conclusion: After long-term moderate-small intensity aerobic exercise, the rats heart had a good adaptive change, and the proteome of left ventricular muscle changed significantly. Long-term moderate-small intensity aerobic exercise can effective enhance the ability of myocardial antioxidation.

Key words: proteome, left-ventricular muscle, moderate-small intensity aerobic exercise, two dimensional gel electrophoresis, mass spectrometry identification

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