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CJAP ›› 2021, Vol. 37 ›› Issue (6): 601-605.doi: 10.12047/j.cjap.6159.2021.095

• ORIGINAL ARTICLES • Previous Articles     Next Articles

Effects of miR-155-3p on the degradation rate of EAF1 mRNA and malignant proliferation in HANK1 cells

LIU Li1△, XU Kai2, WANG Lu-qiang3, XU Mei-li4   

  1. 1. Weifang NO. 2 People's Hospital, Weifang 261041;
    2. Sunshine Union Hospital, Weifang 261000;
    3. Sunshine Union Hospital, Weifang 261000;
    4. Weifang NO. 2 People's Hospital, Weifang 261041, China
  • Received:2020-09-17 Revised:2021-03-19 Online:2021-11-28 Published:2021-11-25

Abstract: Objective: To investigate the effects and mechanisms of miR-155-3p on the malignant behavior of human NK/T cell lymphoma cell line HANK1. Methods: Targetscan database was used to predict the target gene of miR-155-3p. HANK1 cells in logarithmic growth period were cultured, and the cells were divided into blank group, over-expressed group, control group and interference group, which were transfected with pENTER-puro vector, pENTER-miR-155-3p vector, GV248 control vector and GV248-miR-155-3p siRNA interference vector, respectively. Meanwhile, actinomycin D (ActD) was used to treat each group of cells, and the expressions of miR-155-3p, EAf1, β-catenin and c-Myc in each group were detected by real-time fluorescence quantitative PCR (n=5). The degradation rate of EAF1 mRNA, the expressions of EAF1, β-catenin and c-Myc protein were detected by Western blot (n=3), and the malignant proliferation abilities of cells were detected by CCK-8 (n=5). Results: Compared with the blank group, the expression levels of miR-155-3p, β-catenin and c-Myc in the over-expressed group were significantly higher, the expression level of EAF1 was lower, the half-life of EAF1 mRNA was shortened, and the malignant proliferation ability of the cells was strengthened (P<0.05). Compared with the control group, the expression levels of miR-155-3p, β-catenin and c-Myc in the interference group were significantly lower, and the expression level of EAF1 was increased, the half-life of mRNA was prolonged and the ability of cell proliferation was decreased (P<0.05). Conclusion: miR-155-3p can promote EAF1 mRNA degradation and proliferation in HANK1 cells.

Key words: NK/T cell lymphoma, miR-155-3p, EAF1, cell proliferation

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