Effects of three Polyphenolic compounds on the intestinal flora of mice exposed simulated intermittent plateau hypoxia

doi:10.12047/j.cjap.6241.2022.073

Abstract

Abstract: Objective: To investigate the protective effects of three Polyphenolic compounds on intestinal microbial communities in mice exposed intermittent plateau hypoxia. Methods: In this study, 60 healthy male Balb/c mice were randomly divided into plain control group, plateau control group, primary anthocyanin intervention group, quercetin intervention group and resveratrol intervention group, 12 mice in each group. Primary anthocyanin, quercetin and resveratrol were administrated by gavage at the doses of 50, 100 and 20 mg/kg in pharmacological intervention group, respectively. After exposure of the mice to simulation plateau-condition for 30 days, the serum samples were collected for DAO testing, sterile feces were collected in mice, and the diversity and genus level of the mouse gut bacteria were detected by using 16S rRNA technology. Ileum tissue was fixed and stained with HE. Results: HE staining showed that the plateau control group had significant damage to the intestinal tissue structure compared to the plain control group, and the serum DAO concentration was increased (P＜0.05), but there was no statistical difference in the abundance and diversity of intestinal flora species. Contrast to simulated intermittent plateau hypoxia group, the structure of the intestine tissue and the level of DAO in the quercetin intervention group and resveratrol intervention group were improved(P＜0.05), the abundance and α diversity of the intestinal flora were decreased, the relative abundance of Bacteroidetes was reduced(P＜0.05), and the Firmicutes was increased. Concomitantly, significant decreases in relative abundance were observed for Corynebacterium glutamicum and Lactobacillus reuteri(P＜ 0.05). Conclusion: Quercetin and resveratrol showed some degree of protection to mice intestinal microbial communities, and increased the diversity and the abundance of the dominant flora and inhibited the growth of conditional pathogenic bacteria.

Key words: plateau hypoxia, Procyanidin, Quercetin, Resveratrol, intestinal microbial

CLC Number:

R73-3

PAN Cun-yao, ZHANG Bao-yi, LIANG Lan-lan, LIU Hui, GUO Chang-jiang, CHEN Zhao-li, WANG Xin-xing. Effects of three Polyphenolic compounds on the intestinal flora of mice exposed simulated intermittent plateau hypoxia[J]. CJAP, 2022, 38(5): 392-396.

References

[1] Hogberg N, Carlsson PO, Hillered L, et al. Intraluminal intestinal microdialysis detects markers of hypoxia and cell damage in experimental necrotizing enterocolitis[J]. J Pediatr Surg, 2012, 47(9): 1646-1651.
[2] Liu H, Li W, Wang X, et al. Early gut mucosal dysfunction in patients with acute pancreatitis [J]. Pancreas, 2008, 36(2): 192-196.
[3] Bonkowsky JL, Son JH. Hypoxia and connectivity in the developing vertebrate nervous system [J]. Dis Model Mech, 2018, 11(12): 150-154.
[4] Simon MC, Liu L, Barnhart BC, et al. Hypoxia-induced signaling in the cardiovascular system [J]. Annu Rev Physiol, 2008, 70: 51-71.
[5] Gao Z, Wu H, Zhang K, et al. Protective effects of grape seed procyanidin extract on intestinal barrier dysfunction induced by a long-term high-fat diet[J]. J Funct Foods, 2020, 64: 3-7.
[6] 陈樱萌, 胡满江, 曾钰鹏, 等. 槲皮素对肠道菌群的调节作用研究[J]. 食品研究与开发, 2020, 41(10): 6-15.
[7] Zhu F, Zheng J, Xu F, et al. Resveratrol alleviates dextran sulfate sodium-induced acute ulcerative colitis in mice by mediating PI3K/Akt/VEGFA pathway[J]. Front Pharmacol, 2021, 12: 693982.
[8] Guarner F. Role of intestinal flora in health and disease[J]. Nutr Hosp, 2007, 22: 14-19.
[9] Nord CE. The effect of antimicrobial agents on the ecology of the human intestinal microflora[J]. Vet Microbiol, 1993, 35(3-4): 193-197.
[10] Parfenov AI, Bondarenko VM. What we gained from a century of investigations of symbiontic intestinal microflora[J]. Terapevt Arkh, 2012, 84(2): 5-10.
[11] Gao H, Chi X, Li G, et al. Gut microbial diversity and stabilizing functions enhance the plateau adaptability of Tibetan wild ass (Equus kiang)[J]. Microbiologyopen, 2020, 9(6): 10-13.
[12] Zhang J, Zhang J, Wang R, et al. Effects of gut microbiota on drug metabolism and guidance for rational drug use under hypoxic conditions at high altitudes[J]. Curr Drug Metab, 2019, 20(2): 155-165.
[13] 伍荷洁, 张丽萍, 舒青龙. 不同浓度理中汤多糖作为碳源对肠道拟杆菌及厚壁菌体外生长的影响[J]. 山东医药, 2018, 58(23): 29-32.
[14] 张晓梅, 高宇洁, 杨玲, 等. 谷氨酸棒杆菌中氨基酸分泌转运蛋白及其代谢改造研究进展[J]. 生物工程学报, 2020, 36(11): 2250-2259.
[15] 陈晓瑜, 陈峰, 张晨虹. 一株节食小鼠肠道优势鼠乳杆菌加速DSS致慢性结肠炎恢复[J]. 基因组学与应用生物学, 2021, 40(2): 855-866.