Antifatigue effects of the composition of Moringa oleifera leaves and Polygonatum polysaccharide and its mechanisms

doi:10.12047/j.cjap.6307.2022.058

Abstract

Abstract: Objective: To investigate the anti-fatigue effects of composition of Moringa oleifera leaves and Polygonatum polysaccharide, and to explore the mechanisms. Methods: Thirty male Kunming mice were randomly divided into control (C) and composition of Moringa oleifera leaves and Polygonatum polysaccharide group (MP). There were 15 mice in each group. Group C was given distilled water and the group MP was given composition intragastriclly every day. The volume was 0.5 ml. After 14 days of treatment, weight-bearing swimming experiment was conducted, and exhaustive swimming time was recorded. The bearing weight was 3% of the body weight. In another experiment, 48 male Kunming mice were randomly divided into quiet control group (QC), swimming control group (SC) and composition group (MP). There were 16 mice in each group. The QC and SC groups were given distilled water intragastrically, and the group MP was treated with composition every day for 14 days. The volume was 0.5 ml. On the day 15, 30 minutes after intragastriclly administration of distilled water, blood, liver and hind leg muscle of the QC group were collected immediately. The SC and MP groups were subjected non-weight-bearing swimming experiment, and blood, liver and hind leg muscle were collected after swimming. The fatigue related indexes, oxidant/antioxidant parameters and energy metabolism indicators in serum and tissues were determined by commercial kits. Results: The exhaustive swimming time of mice in MP group was significantly longer than that in the C group (P<0.05). Compared with the control group, non-weight-bearing swimming decreased the contents of serum glucose and GSH, the contents of hepatic glycogen and ATP, the hepatic activities of SOD, LDH and ATPase, and muscle activity of GSH-Px (P< 0.05). However, serum levels of BUN and MDA were increased (P<0.05). Compared with the SC group, the composition remarkably increased the contents of serum glucose and hepatic glycogen, increased serum content of GSH, enhanced hepatic activities of SOD, LDH and ATPase and muscle activity of GSH-Px, and increased the hepatic content of ATP (P<0.05). However, the serum level of BUN was decreased (P<0.05). Conclusion: The Moringa oleifera leaves and Polygonatum polysaccharide composition possesses anti-fatigue effects. Anti-oxidant and improving energy metabolism could be the important mechanisms.

Key words: Moringa oleifera leaves, Polygonatum polysaccharide, anti-fatigue, antioxidant, mice

CLC Number:

R151.1

GAO Wei-na, BIAN Xiang-yu, XU Qin-gao, DONG Wei-yun, MA Yu-ying, GUO Chang-jiang. Antifatigue effects of the composition of Moringa oleifera leaves and Polygonatum polysaccharide and its mechanisms[J]. CJAP, 2022, 38(4): 308-312.

References

[1] 张娇, 王元忠, 杨维泽, 等. 黄精属植物化学成分及药理活性研究进展[J]. 中国中药杂志, 2019, 44(10): 1989-2008.
[2] 付莉慧. 滇黄精粗多糖含片制备工艺及其抗疲劳作用的初步研究[D]. 昆明:云南中医药大学, 2019.
[3] 李明. 黄精多糖对运动疲劳大鼠抗氧化及神经递质的影响[J]. 食品科技, 2014, 39(9): 227-230.
[4] Persson PB, Bondke Persson A.Fatigue[J]. Acta Physiol (Oxf), 2016, 218(1): 3-4.
[5] Xie Q, Sun Y, Cao L, et al. Antifatigue and antihypoxia activities of oligosaccharides and polysaccharides from Codonopsis pilosula in mice[J]. Food Funct, 2020,11(7): 6352-6362.
[6] Li W, Luo C, Huang Y, et al. Evaluation of antifatigue and antioxidant activities of the marine microalgae Isochrysis galbana in mice[J]. Food Sci Biotechnol, 2020, 29(4): 549-557.
[7] Powers SK, Nelson WB, Hudson MB.Exercise-induced oxidative stress in humans: cause and consequences[J]. Free Radic Biol Med, 2011, 51(5): 942-950.
[8] Vongsak B, Sithisarn P, Gritsanapan W.Simultaneous HPLC quantitative analysis of active compounds in leaves of Moringa oleifera Lam[J]. J Chromatogr Sci, 2014, 52(7): 641-645.
[9] Waterman C, Rojas-Silva P, Tumer TB, et al. Isothiocyanate-rich Moringa oleifera extract reduces weight gain, insulin resistance, and hepatic gluconeogenesis in mice[J]. Mol Nutr Food Res, 2015, 59(6): 1013-1024.
[10] Atawodi SE, Atawodi JC, Idakwo GA, et al. Evaluation of the polyphenol content and antioxidant properties of methanol extracts of the leaves, stem, and root barks of Moringa oleifera Lam[J]. J Med Food, 2010, 13(3): 710-716.
[11] Sreelatha S, Jeyachitra A, Padma PR.Antiproliferation and induction of apoptosis by Moringa oleifera leaf extract on human cancer cells[J]. Food Chem Toxicol, 2011, 49(6): 1270-1275.
[12] 李欣. 辣木叶黄酮对缓解小鼠运动性疲劳的影响[J]. 食品研究与开发, 2021, 42(8): 38-43.
[13] 王玲玲, 边祥雨, 高蔚娜, 等. 辣木叶总黄酮提取物抗疲劳功效的研究[J]. 营养学报, 2020, 42(1): 68-71.
[14] 刘诗琼, 秦晓群, 李世胜. 黄精多糖对小鼠抗疲劳作用的实验研究[J]. 中国当代医药, 2009, 16(10): 31-32.
[15] 毛雁, 马兰军, 熊正英. 黄精对力竭训练大鼠血清酶活性及某些生化指标的影响[J]. 第四军医大学学报, 2007 (20): 1842-1844.
[16] 石娟, 赵煜, 雷杨, 等. 黄精粗多糖抗疲劳抗氧化作用的研究[J]. 时珍国医国药, 2011, 22(6): 1409-1410.
[17] 王玉勤, 于晓婷, 吴晓岚, 等. 黄精多糖对力竭小鼠脑组织自由基代谢影响[J]. 中国公共卫生, 2014, 30(9): 1165-1167.
[18] Cui X, Wang S, Cao H, et al. A review: The bioactivities and pharmacological applications of polygonatum sibiricum polysaccharides[J]. Molecules, 2018, 23(5): 1170.
[19] 赵士博, 黄锁义, 潘智, 等. 薏苡叶和淫羊藿配伍对小鼠抗疲劳及耐缺氧的作用[J]. 中国应用生理学杂志, 2021, 37(5): 510-513.
[20] 马绍帅, 陈荟宇, 梁芝栋. 决明子水煎液复合 4 周游泳训练对小鼠运动性疲劳的干预作用[J]. 中国应用生理学杂志, 2019, 35(6): 522-524.