Effects of Mijiandao suppository on intestinal laxation in rats and its mechanisms

doi:10.12047/j.cjap.6337.2022.141

Abstract

Abstract: Objective: To investigate the effects of Mijian Daotong Bowel Suppository (MJDs) on the compound diphenoxylate induced constipation model of male rats and its mechanisms. Methods: Sixty SD male rats were randomly divided into blank group, model group, positive group and MJDs group. The constipation model was established by using compound diphenoxylate gavage. The rats in blank group and model group were treated with saline by enema, the rats in positive group and MJDs group were given Kaisailu and honey decoction laxative suppository by enema, respectively, once a day for 10 days. The body weight, fecal water content, gastric emptying rate (GER) and carbon ink propulsion rate (CIPR) of rats were observed during modeling and administration. The effects of MJDs on the pathological changes of colon tissue in constipation rats were investigated by hematoxylin-eosin (HE) staining. The effect of MJDs on 5-hydroxytryptamine (5-HT) in the colon of constipation rats was investigated by ELISA kit. The effects of MJDs on the expressions of aquaporins 3 (AQP3) and aquaporins 4 (AQP4) in the colon of constipation rats were detected by immunohistochemistry. Results: After 10 days of administration, compared with the blank group, the body weight, fecal water content, carbon ink propulsion rate and colon 5-HT content in the model group were decreased significantly, while the expression levels of AQP3 and AQP4 in the colon were increased significantly (P＜0.05, P＜0.01). Compared with the model group, the fecal water content and colon 5-HT content in the positive group were increased significantly, and the expressions of AQP3 and AQP4 in the colon were decreased significantly. The body weight, fecal water content and colon 5-HT content in the MJDs group were increased significantly, and the expressions of AQP3 and AQP4 was decreased significantly (P＜0.05, P＜0.01). Compared with the positive group, the fecal water content of the MJDs group was decreased significantly, and the expressions of AQP3 and AQP4 in the colon of the MJDs group was decreased significantly (P＜0.05, P＜0.01). Gastric emptying rate was not statistically significant difference between the groups. Conclusion: MJDs has good therapeutic effects on constipation, and its mechanisms may be related to up-regulating the content of 5-HT in the colon and down-regulating the expressions of AQP3 and AQP4 in the colon.

Key words: Mijiandao suppository (MJDs), Moisturizing intestines, compound diphenoxylate, aquaporins, 5-hydroxytryptamine (5-HT)

CLC Number:

DU Xiao-feng, CHEN Sheng-fu, SHANG Ting, NI Zhi-tao, SHI Yu-pan, WANG Jin-hui. Effects of Mijiandao suppository on intestinal laxation in rats and its mechanisms[J]. CJAP, 2022, 38(6): 776-781.

References

[1] 陈静, 吕文良. 中医药防治功能性便秘研究进展[J]. 辽宁中医药大学学报, 2021, 23(6): 134-140.
[2] 杨直, 吴晨曦, 高静, 等. 中国成年人慢性便秘患病率的Meta分析[J]. 中国全科医学, 2021, 24(16): 2092-2097.
[3] Guerin A, Mody R, Fok B, et al. Risk of developing colorectal cancer and benign colorectal neoplasm in patients with chronic constipation[J]. Aliment Pharmacol Ther, 2014, 40(1): 83-92.
[4] 贲睿, 孙怡, 李菲菲, 等. 中药靶向治疗气虚型便秘的临床观察[J]. 中国中医药科技, 2022, 29(2): 295-296.
[5] 王向军, 邱雪霏, 苏晓兰, 等. 中药结肠灌洗治疗慢性便秘疗效观察[J]. 中国药业, 2022, 31(9): 105-108.
[6] 商婷, 史玉攀, 倪志涛, 等. 《伤寒论》蜜煎导方的历史沿革及现代临床应用研究[J]. 甘肃医药, 2019, 38(8): 719-722.
[7] 杜晓凤, 张九妹, 商婷, 等. 蜜煎导方治疗功能型便秘的临床疗效[J]. 甘肃医药, 2021, 40(8): 704-705.
[8] 石宇, 于修永, 张虹玺. 助阳通便膏对功能性便秘大鼠肠道酶活性的影响[J]. 中华中医药学刊, 2019, 37(8): 1939-1942.
[9] 何昭璇, 熊静, 王福民, 等. 电针对慢传输型便秘大鼠结肠Cajal间质细胞自噬相关蛋白表达的影响研究[J]. 世界科学技术-中医药现代化, 2021, 23(8): 2928-2933.
[10] 李倩, 吴春芝, 李爽, 等. 中国栓剂的剂型研究与临床应用进展[J]. 华西药学杂志, 2020, 35(6): 691-697.
[11] 王谨慧, 刘萌, 杜晓凤, 等. 新型直肠给药系统及临床应用进展[J]. 甘肃科技, 2019, 35(23): 96-98.
[12] 周晋, 张锋, 张芯, 等. 蜜煎导栓干预气虚津亏型慢性功能性便秘疗效观察[J]. 现代中西医结合杂志, 2017, 26(25): 2751-2753.
[13] 杜丽东, 任远, 牛亭惠, 等. 3种模拟中医体征的体虚便秘大鼠模型建立及效果观察[J]. 中国应用生理学杂志, 2017, 33(2): 140-145.
[14] Mariajoseph-Antony LF, Kannan A, Panneerselvam A, et al. Role of Aquaporins in Inflammation-a Scientific Curation[J]. Inflammation, 2020, 43(5): 1599-1610.
[15] 王煜, 王自立, 王志旺, 等. 润肠通便合剂对便秘模型小鼠结肠MUC2、AQP3的影响[J]. 中国应用生理学杂志, 2022, 38(2): 159-162.
[16] 张欣欣, 刘红宁, 林色奇, 等. 哺乳动物AQPs亚型、分布、功能及中医药调节AQPs防治临床疾病的研究进展[J]. 世界科学技术-中医药现代化, 2022: 1-8.
[17] 杜丽东, 任远, 吴国泰, 等. 当归对实验性血虚便秘模型小鼠结肠组织形态和黏液分泌的影响[J]. 中国应用生理学杂志, 2018, 34(4): 367-370.
[18] Israelyan N, Del CA, Li Z, et al. Effects of serotonin and slow-release 5-hydroxytryptophan on gastrointestinal motility in a mouse model of depression[J]. Gastroenterology, 2019, 157(2): 507-521.
[19] 陈振海, 冯江毅, 胡淞, 等. 枯草芽孢杆菌通过TGR5/TRPA1信号通路调节慢性传输型便秘小鼠的肠蠕动[J]. 中国现代应用药学, 2022, 39(7): 878-884.
[20] 朱依琳, 张建军, 汤如莹, 等. 芦荟与火麻仁配伍对复方地芬诺酯致便秘小鼠的影响[J]. 中华中医药杂志, 2021, 36(8): 4986-4990.