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CJAP ›› 2016, Vol. 32 ›› Issue (2): 116-120.doi: 10.13459/j.cnki.cjap.2016.02.006

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Effect of NADPH oxidase inhibitor apocynin on the exercise induced proteinuria in rats

CHEN Li-na, ZHOU Gang, WU le, HOU Shao-hua   

  1. College of Sport, Hunan University, Changsha 410082, China
  • Received:2015-05-25 Revised:2015-11-11 Online:2016-03-28 Published:2018-06-12
  • Supported by:
    湖南省自然科学基金资助(12JJ3093)

Abstract: Objective: To investigate the effect of NADPH oxidase inhibitor apocynin on exercise-induced proteinuria and its related mechanism. Methods: Thirty-two SD rats were randomly divided into the control group (group C), control + drug group (group CA), exhaustive exercise group (group E), exhaustive exercise + drug group (group EA). The rats were administrated apocynin at 10 mg/kg weight,once a day for three days, and one hour after the drug injection, a one-time exhaustive exercise was performed. After exhaustive exercise, urine protein, blood urea nitrogen (BUN), and kidney reactive oxygen species (ROS) concentration, NOS activity, NOS and 3-NT concentration were detected. Results: In comparison to control group urinary protein (UP), ROS, inductible nitric oxide synthase (iNOS), 3-NT levels increased significantly in group E while those in group EA did not change. Conclusion: The elevated renal NADPH oxidase activity by exhaustive exercise induced ROS that can rapidly react with NO, and then produces excess peroxynitrite, which contributes to occurrence of exercise-induced proteinuria.

Key words: NADPH oxidase, exercise-induced proteinuria, reactive oxygen species (ROS), peroxynitrite (ONOO-), nitric oxide

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