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CJAP ›› 2016, Vol. 32 ›› Issue (2): 128-131.doi: 10.13459/j.cnki.cjap.2016.02.009

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Effects of docosahexaenoic acid on atrial physiological parameter and TASK-1 protein expression in atrial fibrillation rats

GE Min1, SHAN Feng1,2, TANG Bi3, HOU Xiu-Jie4   

  1. 1. Department of Pharmacy, Bengbu Medical College, Bengbu 233004;
    2. Department of Pharmacy, the Fourth People's Hospital of Hefei, Hefei 230022;
    3. Department of Cardiovascular Medcine, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233004;
    4. Department of Cardiovascular Medcine, the First People's Hospital of Fuyang, Fuyang 236000, China
  • Received:2015-05-11 Revised:2015-11-27 Online:2016-03-28 Published:2018-06-12
  • Supported by:
    国家自然科学基金资助项目(81170046);教育部留学回国人员科研启动基金(第46批);安徽省自然科学基金资助项目(10040606Q44)

Abstract: Objective: To investigate the effect of docosahexaenoic acid (DHA) on atrial physiological parameter and its related mechanisms in atrial fibrillation rats. Methods: Eighty Sprague-Dawley (SD) rats which were sensitive to acetylcholine-calcium chloride mixture were randomly divided into four groups:control (CTL), control treated with DHA (DHA), atrial fibrillation (AF) and atrial fibrillation treated with DHA (DHA+AF). The duration of atrial fibrillation was measured. The atrial myocyte action potential duration (APD) and TWIK-related acid-sensitive K+ channels-1 (TASK-1) currents were recorded by whole-cell patch-clamp technique. The expression of TASK-1 at protein level in atrial tissue was detected by Western blot method. Results: Atrial fibrillation of the rats was induced by acetylcholine-calcium chloride mixture, and the duration time of atrial fibrillation was increased with the drug-induced time prolonged. Compared with control group, the time of atrial myocyte action potential duration at 50% repolarization (APD50) and at 90% repolarization (APD90) were significantly shorten in AF group, TASK-1 current density and TASK-1 protein expression were increased (P<0.05). Compared with AF group, the duration of atrial fibrillation was decreased,the time of atrial myocyte APD50 and APD90 were prolonged, TASK-1 current density and protein expression were significantly reduced in DHA+AF group (P<0.05). Conclusion: DHA can prolong the atrial myocyte APD in atrial fibrillation rats, which may be related to down-regulation of TASK-1 protein expression and decreasing TASK-1 current density in atrial tissue.

Key words: atrial fibrillation, docosahexaenoic acid, atrial electrical remodeling, TASK-1

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