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CJAP ›› 2016, Vol. 32 ›› Issue (2): 154-157.doi: 10.13459/j.cnki.cjap.2016.02.015

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The effect of astragalus injection on the expression of endoplasmic reticulum stress chaperonin GRP78 and GRP94 mRNA in adriamycin-injured cardiomyocytes with calumenin silencing by shRNA

MA Kui-ying, WAN Quan, WANG Yi-li, TAO Xie-xin, HUANG Xia, CHAI Hua, ZHAO Ming   

  1. The Inner-Mongolia National University Affiliated Hospital, Tongliao 028000, China
  • Received:2014-12-10 Revised:2015-11-02 Online:2016-03-28 Published:2018-06-12
  • Contact: 赵明,Tel:13474859991;E-mail:langzhe73@163.com E-mail:langzhe73@163.com
  • Supported by:
    赵明,Tel:13474859991;E-mail:langzhe73@163.com

Abstract: Objective: To investigate the effect of astragalus injection on the endoplasmic reticulum stress in adriamycin(ADR)-injured cardiomyocytes with calumenin silencing by shRNA. Methods: Firstly, the stable lentiviral calumenin shRNAvector was constructed. Secondly, in vitro cultured neonatal rat cardiac myocytes were randomly divided into control group、normal group (3 mg/L ADR), lentivirus infection group (lentivirus infection+3 mg/L ADR), Astragalus group 1 (3 mg/L ADR+Astragalus), Astragalus group 2 (lentivirus infection+3 mg/L ADR+Astragalus). The mRNA epression level of calumenin expression and reticulum stress chaperone in GRP78, GRP94 of each group was monitored by real-time PCR. Results: ①Compared with that of the control group, the calumenin mRNA expression in the normal group was reduced(P<0.05), yet its mRNA level in lentivirus the infection group and the Astragalus group 2 was further reduced(P<0.01). Compared with that of the normal group, the mRNA contents of calumenin in the Astragalus group 1 was increased(P<0.05). The expression of calumenin in Astragalus group 2 was increased comparing with lentivirus infection group (P<0.01). ②Compared with that in the control group, the expression of reticulum stress chaperone in GRP78, GRP 94 in lentivirus infection group and normal group was significantly increased (P<0.01); Compared with that in the normal group, the expression of reticulum stress chaperone in GRP78, GRP94 in Astragalus group 1 was reduced(P<0.01); Compared with that in the lentivirus infection group, the expression of reticulum stress chaperone in GRP78, GRP94 in the Astragalus group 2 was obviously decreased(P<0.01). Conclusion: ①Calumenin can alleviate endoplasmic reticulum stress induced by ADR-injured myocardial cells. ②Astragalus injection can restrain the endoplasmic reticulum stress induced by adriamycin, which may be achieved by the calumenin protein.

Key words: adriamycin, astragalus, calumenin, endoplasmic reticulum stress, cardiomyocyte, suckling rat, eticulum stress, cardiomyocyte, suckling rat

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