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CJAP ›› 2016, Vol. 32 ›› Issue (3): 274-277.doi: 10.13459/j.cnki.cjap.2016.03.022

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Effects of activation of dopamine type I receptor on the produc-tion of NO/NOS in ox-LDL activated THP-1 cells

LIU Lei, SHI Sa, LI Hong-zhu, LI Hong, XU Chang-qing   

  1. Department of Pathophysiology, Harbin Medical University, Harbin 150086, China
  • Received:2015-03-09 Revised:2015-10-20 Online:2016-05-28 Published:2018-06-12
  • Supported by:
    黑龙江省自然科学基金项目资助(D200880);黑龙江省教育厅课题(12521181)

Abstract: Objective:To study the effect of excited dopamine type I receptor on the production of nitric oxide/nitric oxide synthase(NO/NOS)in ox-LDL activated THP-1 cells and the possible mechanism. Methods:Cultured THP-1 cells activated by PMA were randomly assigned in the following groups:control group (control), oxidized low density lipoprotein group (ox-LDL), dopamine receptor 1(DR1) agonist group (SKF), DR1 antagonist group (SCH), ERK blocker group (PD98059). Oil Red O staining was used to identify the accumulation of cellular lipid. The levels of NO and NOS in the supernatant of THP-1 were assayed by nitrate reductase method. The protein expression of DR1, p-ERK and ERK were obtained by Western blot and immunity fluorescence. Results:After 48 h of incubation of ox-LDL, accumulation of lipid in the cytoplasm was found in most THP-1 cells. Compared with control group, DR1 protein expression was reduced in ox-LDL-induced cells(P<0.01). Activation of DR1 agonist decrease the production of NO and iNOS(P<0.01), and PD98059 partly reversed the above effect. Conclusion:Activation of DR1 can inhibit the production of NO/NOS in ox-LDL-induced THP-1 cells, which may be related with ERK pathway.

Key words: dopamine receptor(DR), foam cells, nitric oxide(NO), THP-1 cells, LDL

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