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CJAP ›› 2016, Vol. 32 ›› Issue (4): 301-304.doi: 10.13459/j.cnki.cjap.2016.04.004

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The effects of prenatal stress on the astrocytes after cerebral ischemia/reperfusion injury in adult offspring rats

WANG Ling-xing, HUANG Hong-hong, CHEN Ya-fang, CAI Hong-chao, QIAN Jia-qiang   

  1. Department of Neurology, the Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China
  • Received:2015-11-24 Revised:2016-04-04 Online:2016-07-28 Published:2018-06-20
  • Supported by:
    福建省自然科学基金项目(2015J01449);福建省教育厅项目(JB13067);泉州市技术研究与开发项目(2012Z35);院苗圃基金(2012MP65)

Abstract: Objective:To investigate the effects of prenatal stress on astrocytes after ischemia/reperfusion of cerebral middle artery in adult offspring rats. Methods:Pregnant rats were randomly assigned to prenatal stress treatment group, which was exposed to restraint three times daily in the last week of pregnancy, and no prenatal stress treatment group. Adult male offspring rats were subjected to transient focal cerebral ischemia by middle cerebral artery occlusion (MCAO). There were three groups:prenatal stress+sham group, MCAO group and prenatal stress+MCAO group (n=10). After 5 days of reperfusion, the infarct size was evaluated. The morphology of astrocytes, co-local-ization of erythropoietin-producing hepatocellular receptor A4 (EphA4) and glial fibrillary acidic protein (GFAP) were detected by double im-munofluorescent staining. And the protein expressions of EphA4, GFAP and Neurocan in peri-ischemic regions were detected by Western blot. Results:The infarct size and the expression of EphA4, GFAP and Neurocan were significantly increased in prenatal stress+MCAO group compared with MCAO group (all P<0.05). And the morphological changes of GFAP-positive astrocytes and co-localization of EphA4/GFAP were more obvious in prenatal stress+MCAO group compared with MCAO group. Conclusion:Prenatal stress may upregulate the expression of EphA4 on astrocytes in the offspring rats after cerebral ischemia/reperfusion, which promotes the reactivity of astrocyte and increases the ex-pression of neurocan.

Key words: prenatal stress, offspring rats, cerebral ischemia, astrocyte

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