Effects of low-concentrations alcohol consumption on the expression of mitofusin-2 in myocardial injury of diabetic rats

doi:10.13459/j.cnki.cjap.2016.04.016

CJAP ›› 2016, Vol. 32 ›› Issue (4): 347-350.doi: 10.13459/j.cnki.cjap.2016.04.016

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Effects of low-concentrations alcohol consumption on the expression of mitofusin-2 in myocardial injury of diabetic rats

KANG Pin-fang¹, HU Jun-feng², TANG Yang¹, YE Hong-wei³, GAO Qin³, WU Wen-juan⁴, TANG Bi¹, ZHANG Heng¹, WANG Hong-ju¹

1. Department of Cardiovascular Medicine, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233004;
2. Department of Respiratory Medicine, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233004;
3. Department of Physiology, Bengbu Medical College, Bengbu 233030;
4. Department of Biochemistry and Molecular, Bengbu Medical College, Bengbu 233030, China

Received:2016-01-08 Revised:2016-04-18 Online:2016-07-28 Published:2018-06-20
Supported by:
国家自然科学基金资助项目（81550036，81000074）；安徽省自然科学基金资助项目（1508085MH169）；蚌埠医学院科技发展基金资助项目（Bykf13A16）；教育部回国留学人员启动基金资助项目（第46批-教外司留[2013]693号）

Abstract

Abstract: Objective:To observe the effects of low-concentrations of alcohol consumption on the expression of mitofusin-2 (mfn2) in myocardial injury of diabetic rats. Methods:Diabetic rat model was simulated by intraperitoneal injection of 55 mg/kg streptozotocin (STZ) and divided into control group, diabetes mellitus(DM) and diabetes+ethanol (DM+EtOH) groups (n=6). When diabetic model was suc-ceed, daily consumption of 2.5% ethanol was used in ethanol+diabetic group after one week, then changed to 5% ethanol continued until 8 weeks. Eight weeks after the modeling, heart perfusion ex vivo. The ventricular hemodynamic parameters were recorded, the serum levels of lactate dehydrogenase (LDH) and aspartate aminotransferase (AST) were determined by automatic biochemistry analyzer, the mfn2 protein ex-pression of left anterior myocardium was evaluated by Western blot and immunohistochemistry. Results:Compared with control group, the left ventricular development pressure (LVDP), heart rate (HR) and rate pressure product (RPP) were decreased, however, left ventricular and diastolic pressure (LVEDP) and LDH, AST release were increased, the expression of mfn2 protein was decreased in DM group. Compared with DM group, LVDP, HR and RPP and the expression of mfn2 protein were increased, LVEDP and LDH, AST were decreased in DM+E-tOH group. Conclusion:The expression of mfn2 protein was decreased in myocardial injury of diabetic rats, low-concentrations of alcohol consumption increased the expression of mfn2. It suggests that mfn2 may be participated in the cardiac protective role of low-concentrations al-cohol intervene in diabetic rat.

Key words: mitofusin-2, diabetes mellitus, myocardial injury, low-doses of alcohol, rat

KANG Pin-fang, HU Jun-feng, TANG Yang, YE Hong-wei, GAO Qin, WU Wen-juan, TANG Bi, ZHANG Heng, WANG Hong-ju. Effects of low-concentrations alcohol consumption on the expression of mitofusin-2 in myocardial injury of diabetic rats[J]. CJAP, 2016, 32(4): 347-350.

References

[1] Yuan Q, Hong S, Han S, et al. Preconditioning with physi-ological levels of ethanol protect kidney against ischemia/reperfusion injury by modulating oxidative stress[J]. PLoS One, 2011, 6(10):e25811.
[2] 王洪巨, 康品方, 叶红伟, 等. 激动乙醛脱氢酶2对抗糖尿病大鼠心肌缺血/再灌注损伤的作用[J]. 中国应用生理学杂志, 2012, 28(2):133-137.
[3] 于影, 康品方, 李徽徽, 等. 乙醇激动ALDH2对糖尿病大鼠肾脏JNK表达的影响[J]. 中国应用生理学杂志, 2014, 30(3):270-273.
[4] Ong SB, Kalkhoran SB, Cabrera-Fuentes HA, et al. Mito-chondrial fusion and fission proteins as novel therapeutic tar-gets for treating cardiovascular disease[J]. Eur J Pharma-col, 2015, 763(pt A):104-114.
[5] 汪华学, 陶静, 叶红伟, 等. Β-catenin和WISP-1在糖尿病大鼠心肌中的表达[J]. 中国组织化学与细胞化学杂志, 2013, 22(3):189-192.
[6] Peng HC, Chen YL, Yang SY, et al. The antiapoptotic ef-fe cts of different doses of β-carotene in chronic ethanol-fed rats[J]. Hepatobiliary Surg Nutr, 2013, 2(3):132-141.
[7] Li SY, Li Q, Shen JJ, et al. Attenuation of acetaldehyde-in-duced cell injury by overexpression of aldehyde dehydroge-nase-2(ALDH2) transgene in human cardiac myocytes:role of MAP kinase signaling[J]. J Mol Cell Cardiol, 2006, 40(2):283-294.
[8] Goral J, Karavitis J, Kovacs EJ. Exposure-dependent effects of ethanol on the innate immune system[J]. Alcohol, 2008, 42(4):237-247.
[9] Krenz M, Korthuis RJ. Moderate ethanol ingestion and car-diovascular protection:From epidemiologic associations to cellular mechanisms[J]. J Mol Cell Cardiol, 2012, 52(1):93-104.
[10] Facchini FS, Saylor KL. A low-iron-available, polyphenol-enriched, carbohydrate-restricted diet to slow progression of diabetic nephropathy[J]. Diabetes, 2003, 52(5):1204-1209.
[11] Elmadhun NY, Sabe AA, Lassaletta AD, et al. Alcohol consumption mitigates apoptosis and mTOR signaling in my-ocardium[J]. J AM Coll Surg, 2014, 218(6):1175-1181.
[12] Lassaletta AD, Cheung EC, Neuspiel M, et al. Ethanol pro-motes arteriogenesis and restores perfusion to chronically is-chemic myocardium[J]. Circulation, 2013, 128(11 suppl 1):S136-S143.
[13] 郭艳红, 李黔, 于海奕, 等. 增殖抑制基因诱导平滑肌细胞的凋亡[J]. 北京大学学报(医学版), 2007, 39:394-398.
[14] Jahani-Asl A, Cheung EC, Neuspiel M, et al. Mitofusin 2 protects cerebellar granule neurons against injury-induced cell death[J]. J Biol Chem, 2007, 282(33):23788-23798.
[15] 陈春蕾, 沈涛, 郑铭, 等. 线粒体融合蛋白Mfn2对心肌细胞肥大的抑制作用[J]. 北京大学学报(医学版), 2008, 40(5):528-532.
[16] Gao Q, Wang XM, Ye HW, et al. Changes in the expres-sion of cardiac mitofusin-2 in different stages of diabetes in rats[J]. Mol Med Rep, 2012, 6(4):811-814.

Effects of low-concentrations alcohol consumption on the expression of mitofusin-2 in myocardial injury of diabetic rats

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