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CJAP ›› 2016, Vol. 32 ›› Issue (4): 370-372.doi: 10.13459/j.cnki.cjap.2016.04.021

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Effect of arecoline on proliferation and apoptosis of MCF-7 human breast cancer cells

FENG Shui-dong1, WU Di2, YANG Si-si2, HE Jian-qin2, ZHANG Kai-fang2, LING Hong-yan2   

  1. 1. Department of Social Medicine and Health Service Management, School of Public Health, Hengyang 421001, China;
    2. Department of Physiology, School of Medicine, University of South China, Hengyang 421001, China
  • Received:2015-06-08 Revised:2016-02-29 Online:2016-07-28 Published:2018-06-20
  • Supported by:
    教育部留学归国基金(教外司留[2014]1685号);中国博士后科学基金(2012M511384);湖南省科技厅(2015JC3085)项目资助;南华大学留学归国基金项目(2013XQD49)

Abstract: Objective:To observe the effects of arecoline on proliferation and apoptosis of MCF-7 human breast cancer cells and to ex-plore its possible mechanism. Methods:Human breast cancer MCF-7 cells were treated with arecoline at the concentrations of 0,10,30,50, 100,300,500μmol/L, the cell proliferation were detected by MTT assay, cell apoptosis were analyzed by Hoechst 33342 staining and flow cy-tometry, the protein expression of Bax,Bcl-2 and P53 were detected by Western blot. Results:Low concentration(0,10,30, 50 μmol/L) arecoline had no effect on the proliferation and apoptosis of MCF-7. However, high concentration(100,300,500μmol/L) arecoline inhibited proliferation and induced apoptosis of MCF-7 cells in a concentration-dependent manner, arecoline also significantly increased P53 and Bax protein expression and decreased Bcl-2 protein expression. Conclusion:High concentration arecoline inhibited the proliferation and induced the apoptosis of MCF-7 cells, the mechanism was probably corrected with increasing P53 and Bax protein expression and decreasing Bcl-2 pro-tein expression.

Key words: arecoline, MCF-7 cells, proliferation, apoptosis

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