Effect of NADPH oxidase inhibitor apocynin on the exercise induced proteinuria in rats

doi:10.13459/j.cnki.cjap.2016.02.006

Abstract

Abstract: Objective: To investigate the effect of NADPH oxidase inhibitor apocynin on exercise-induced proteinuria and its related mechanism. Methods: Thirty-two SD rats were randomly divided into the control group (group C), control + drug group (group CA), exhaustive exercise group (group E), exhaustive exercise + drug group (group EA). The rats were administrated apocynin at 10 mg/kg weight,once a day for three days, and one hour after the drug injection, a one-time exhaustive exercise was performed. After exhaustive exercise, urine protein, blood urea nitrogen (BUN), and kidney reactive oxygen species (ROS) concentration, NOS activity, NOS and 3-NT concentration were detected. Results: In comparison to control group urinary protein (UP), ROS, inductible nitric oxide synthase (iNOS), 3-NT levels increased significantly in group E while those in group EA did not change. Conclusion: The elevated renal NADPH oxidase activity by exhaustive exercise induced ROS that can rapidly react with NO, and then produces excess peroxynitrite, which contributes to occurrence of exercise-induced proteinuria.

Key words: NADPH oxidase, exercise-induced proteinuria, reactive oxygen species (ROS), peroxynitrite (ONOO^-), nitric oxide

CHEN Li-na, ZHOU Gang, WU le, HOU Shao-hua. Effect of NADPH oxidase inhibitor apocynin on the exercise induced proteinuria in rats[J]. CJAP, 2016, 32(2): 116-120.

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