Effects of acute cold exposure on the expression of AQP-1 and AQP-5 in rat lung tissues

doi:10.13459/j.cnki.cjap.2016.03.012

Abstract

Abstract: Objective:To study the effect of acute cold exposure on the expression of aquaporin-1 (AQP-1) and AQP-5 in lung tissues and the changes of ultrastructural pathological changes after cold exposure in rats. Methods:Twelve male Wistar rats were randomly divided into control group (23±2)℃ and cold (-25℃) exposure group, the exposure time was 2 h. Rectal temperatures of the rats were measured immediately after cold exposure. The ultrastructural pathological changes of pulmonary tissue were observed by transmission electronic microscope. The mRNA expression of AQP-1 and AQP-5 was measured by RT-PCR. The protein expression of AQP-1 and AQP-5 was measured by Western blot. Results:The body core temperatures(28.07±4.15)℃ of the cold exposure group were decreased significantly compared with the control group(37.33±0.25)℃ (P<0.05). In acute cold exposure group, the main pathological changes of pulmonary ulstructure included pulmoary epithelial basement membrane thickening, and karyopyknosis of AT-I cells, and vacuolization on the cytoplasm of (AT-Ⅱ) cells. After acute cold exposure, the levels of both mRNA and potein expressions of AQP-5 were decreased significantly (P<0.05) compared with those in the control group. while AQP-1 expression level showed no statistical significance between control group and cold exposure group. Conclusion:There might be some cause and effect relationship between lung tissue damage by cold exposure and the levels of mRNA and potein expressions of AQP-5 decreased after acute cold exposure.

Key words: acute cold exposure, lung, aquaporin, rat

LIN Yang-sheng, ZHANG Li, YANG Hua, ZHANG Ying, LI Xi, ZHANG Yong-qiang, YANG Dan-feng. Effects of acute cold exposure on the expression of AQP-1 and AQP-5 in rat lung tissues[J]. CJAP, 2016, 32(3): 234-237.

References

[1] D'Amato G, Cecchi L, D'Amato M, et al. Urban air pollution and climate change as environmental risk factors of respiratory allergy:An Update[J]. J Invest Allergol Clin Immunol, 2010, 20(2):95-102.
[2] Kim H, Heo J, Kim H, et al. Has the impact of temperature on mortality really decreased over time[J]. Sci Total Environ, 2015, 512:74-81.
[3] Zhou XD, Zhao A, Meng X, et al. Acute effects of diurnal temperature range on mortality in 8 Chinese cities[J]. Sci Total Environ, 2014, 493:92-97.
[4] Khadadah M, Mustafa S, Elgazzar A. Effect of acute cold exposure on lung perfusion and tracheal smooth muscle contraction in rabbit[J]. Eur J Appl Physiol, 2011, 111(1):77-81.
[5] Verkman AS. Aquaporin water channels and endothelial cell function[J]. J Anat, 2002, 200(6):617-627.
[6] Towne JE, Harrod KS, Krane CM, et al. Decreased expression of aquaporin (AQP)1 and AQP5 in mouse lung after acute viral infection[J]. Am J Respir Cell Mol Biol, 2000, 22(1):34-44.
[7] Sun CY, Zhao YX, Zhong W, et al. The expression of aquaporins 1 and 5 in rat lung after thoracic irradiation[J]. J Radiat Res, 2014, 55(4):683-689.
[8] Mäkinen TM, Hassi J. Health Problems in Cold Work[J]. Ind Health, 2009, 47(3):207-220.
[9] Itoh T, Rai T, Kuwahara M, et al. Identification of a novel aquaporin, AQP12 expressed in pancreatic acinar cells[J]. Biochem Biophys Res Commun, 2005, 330(3):832-838.
[10] Towne JE, Harrod KS, Krane CM, et al. Decreased expression of aquaporin (AQP)1 and AQP5 in mouse lung after acute viral infection[J]. Am J Respir Cell Mol Biol, 2000, 22(1):34-44.
[11] Towne JE, Krane CM, Bachurski CJ, et al. Tumor necrosis factor-alpha inhibits aquaporin 5 expression in mouse lung epithelial cells[J]. J Biol Chem, 2001, 276(22):18657-18664.
[12] 李波, 陈东, 王桂芳, 等. 水通道蛋白1、3、4、5在内毒素性急性肺损伤小鼠肺组织中的表达[J]. 第二军医大学学报, 2008, 29(2):131-135.

[1]	HU Jing, LI Fa-dong, SHAO Xiao-ting, LI Si-wei, ZHANG Xin-ying, ZHAO Bing-bing, XU Chang-qing, WEI Can. Spermine attenuates high glucose-induced myocardial fibrosis by regulating the cell cycle [J]. CJAP, 2020, 36(3): 193-196.
[2]	ZHU Jian-zhong, ZHAO Can, SUI Yue-lin, LIU Yuan-yuan, QIAO Yue-bing. Effects of leptin on lipid metabolism and inflammatory factors in diabetic rats [J]. CJAP, 2020, 36(3): 197-201.
[3]	SUN Xiao-juan, FENG Wu-long, HOU Na, LI Na, JIANG Wei-li. Effects of aerobic exercise and resveratrol on the expressions of JAK2 and TGF-β1 in renal tissue of type 2 diabetes rats [J]. CJAP, 2020, 36(3): 202-206.
[4]	LIU Kai-li, DU Xin-xin, ZHANG Wen-qin, WU Ya-li, WANG Xi, WANG Chun-fang, CUI Xiang-li. Overexpression of miR-31 regulates TLR4/NF-κB signaling pathway and apoptotic protein in colitis model mice [J]. CJAP, 2020, 36(3): 211-215.
[5]	SHI Hui, LIANG Xiao-shan, HUANG Li-wen, LUO Zhi-gang, TAN Long. The optimization and assessment of the method for inducing hyperuricemia in rats [J]. CJAP, 2020, 36(3): 223-227.
[6]	HAN Jie, LIU Zi-yi, FANG Zhen, TIAN Lei, YANG Dan-feng, XI Zhu-ge, LIU Xiao-hua. Effects of repeated immobilization stress on hypothalamic-pituitary- ovarian axis in female rats [J]. CJAP, 2020, 36(3): 245-249.
[7]	CHEN Yu-feng, DONG Fan-he, LOU Yun-wei, SHOU Jin-hao, ZHANG Hui-ting, ZHOU Yi-chao, YAN Ming, MAO Hong-jiao, ZHANG Yun. Effect of psoralen on rat osteoblasts injuries induced by TCP wear particles in vitro and its mechanism [J]. CJAP, 2020, 36(3): 255-260.
[8]	. [J]. CJAP, 2020, 36(3): 265-267.
[9]	XU Chang-shun, SUN Pei, LIN Chun. A new design puncture needle and a device of microcatheter protection for lumbar intrathecal catheterization in rats [J]. CJAP, 2020, 36(3): 283-288.
[10]	WANG Ling-xing, HONG Shan-yan, YANG Mei-li, HUANG Hong-hong. Effects of butylphthalide on microglia activation in frontal lobe of rats after chronic sleep deprivation [J]. CJAP, 2020, 36(2): 106-110.
[11]	WAN Jian-yong, ZHANG Yong, LIU Shi-qiang, HUA Tian-miao, SUN Qing-yan. Effects of exercise intervention on BIM signaling in liver of insulin resistance rats [J]. CJAP, 2020, 36(2): 115-118.
[12]	JIN Zhao, CAO Zheng-tao, WANG Hai-xia, ZHANG Li-hui, LI Yi-feng, ZHOU Yu-bin, WANG Quan, LI Bao-hui, XU Yan, WEI Xiao-yang, WANG Hong, LIN Rong, YANG JING-hui, GENG Xi-chen. Changes of regional cerebral oxygen saturation under gradual-onset rate +Gz exposure [J]. CJAP, 2020, 36(2): 130-133.
[13]	ZHOU Bi-ye, LI Jing, LI Ming-gao, SUN Hai-wen, ZHOU Jia-xing, HAN Lei. Effect of repeated horizontal acceleration exposure on cardiac structure in New Zealand rabbits [J]. CJAP, 2020, 36(2): 134-137.
[14]	YAO Liang-xue, HAN Jia-xin, WEI Pei-yun, HOU Si-bo, HE Xiao-jian, ZHANG Rui-ling, DU Ai-lin. Effects of butylphthalide on H₂S/CBS pathway in hippocampal and amygdala and learning and memory ability in chronic alcoholism rats [J]. CJAP, 2020, 36(2): 138-142.
[15]	LIANG Wan-ling, XIAO Tian-bao, YUAN Feng, CAO Yi-bo, YAN Deng-guo. Effects of microRNA95 targeting FOXD1 gene on radiosensitivity of colorectal cancer loVo cells and its mechanism [J]. CJAP, 2020, 36(2): 148-151.