The effect of inhibition of hyperplasia on spinal cord reactive astrocytes by mild hypothermia

doi:10.13459/j.cnki.cjap.2016.04.001

Abstract

Abstract: Objective:To observe changes in the morphology and activity of astrocytes in spinal cord at different temperatures after scratches treatment, and to investigate the effect of mild hypothermia on hyperplasia of reactive astrocytes. Methods:Spinal cord astrocytes were cultured from the neonatal SD rat, and reactive astrocytes were prepared by scratches treatment. Mild hypothermia choose 33℃, cell cul-ture 48 h. Cells were divided into control group、scratches group、mild hypothermia group and scratch+mild hypothermia group. Cell mor-phology of each group were observed in 0 h、6 h、12 h、24 h、48 h、3 d、5 d、7 d. Nestin positive rate were detected by using immunofluorescence staining method. Cell activity were observed by methyl thiazolyl tetrazolium salt (MTT) assay. The degree of apoptosis was observed by using PI staining method. Results:Compared with control group and mild hypothermia group, cell body in both scratches group and scratch+mild hypothermia group was hypertrophy, the protrusion around was increased and extend, the cytoplasm was enrich, and the growth rate was signif-icantly increased. Compared with scratches group, scratch+mild hypothermia group cells grew slowly, the protrusion around was decreased, and cells of the scratched area ingrowth significantly slowly. Nestin and PI positive rate of cells were also significantly lower. All the results had statistically significant differences (P<0.01). Conclusion:After scratch injury, astrocytes were activated to be reactive astrocytes and hyper-plasia. Mild hypothermia significantly inhibited spinal cord reactive astrocytes overgrowth and restrain astrocytes apoptosis.

Key words: mild hypothermia, spinal cord injury, rats

KANG Wen-bo, LI Xiao-hong, CHEN Chong, WANG Jing-jing, TU Yue, ZHANG Sai, LIANG Hai-qian. The effect of inhibition of hyperplasia on spinal cord reactive astrocytes by mild hypothermia[J]. CJAP, 2016, 32(4): 289-292.

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