Effects of supplement of corn peptides combined with aerobic exercise on lipolysis key enzymes:adipose triglyceride lipase and lipoprotein lipase of obese rats

doi:10.13459/j.cnki.cjap.2016.04.010

Abstract

Abstract: Objective:To investigate the effects of supplement of corn peptides plus aerobic exercise on fat loss and blood lipid profile in obese rats resulted from high fat diet and the mechanism:the role of adipose triglyceride lipase (ATGL) and lipoprotein lipase (LPL). Methods:One hundred and fifty male SD rats (4 weeks age) were randomly divided into control group (C, n=15) fed with ordinary diet and obese model group (M, n=135) fed with high fat diet for 8 weeks. Forty obese rats whose body weight increased by 20% of the mean value of con-trol group were selected and randomly divided into 5 groups(n=8):obesity control group, casein group, corn peptides group, exercise group and exercise+corn peptides group. The rats of the latter two groups completed aerobic excise for 4 weeks at speed of 15 m/min and duration of 60 min per time (6 times/week). After 4 weeks of intervention, the bloods of rats were collected and blood lipid profile plasma levels of triglyceride(TG), total cholesterol (TC), high density lipoprotein(HDL), low density lipoprotein(LDL) were detected. The perirenal fat and epididymal fat were collected and weighed, and the protein levels of ATGL in livers and LPL in adipose tissues were detected by Western blot. Results:①Compared with obesity control group, the body weight and the masses of perirenal fat and epididymal fat in exercise group and corn peptides+exercise group were decreased obviously, with more obvious decrease in the corn peptides+exercise group compared with the exer-cise group. No difference was found in the rats of corn peptides group and casein group. ②Compared with obesity control group, the plasma TG was decreased in exercise group, and the plasma levels of TG and TC were reduced in the rats of corn peptides+exercise group, while they remained unchanged in the rats of corn peptides group and casein group. The plasma levels of HDL and LDL had no significant difference a-mong groups. ③The protein levels of ATGL in livers and LPL in adipose tissues were significantly increased in the rats of exercise group and corn peptides+exercise group, with more obvious in the latter group. No difference was shown in the rats of corn peptides group and casein group compared with obesity control group. Conclusion:Significant decrease of body weight, perirenal fat and epididymal fat as well as plasma TG, TC were induced by 4-week aerobic exercise or supplement of corn peptides plus exercise, with more obvious effects induced by corn pep-tides plus exercise than exercise, while no effect was induced by corn peptides alone, which may be related to the enhancements of the protein levels of ATGL in livers and LPL in adipose tissues.

Key words: corn peptides, aerobic exercise, ATGL, LPL, blood lipid profile

LU Lin, LIU Gui, WANG Xiao-hui, LOU Shu-jie. Effects of supplement of corn peptides combined with aerobic exercise on lipolysis key enzymes:adipose triglyceride lipase and lipoprotein lipase of obese rats[J]. CJAP, 2016, 32(4): 326-331.

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