The expression of calcium sensing receptor(CaSR) and MAPK pathway changes in myocardial cells of epilepsy rats

doi:10.13459/j.cnki.cjap.2016.05.018

CJAP ›› 2016, Vol. 32 ›› Issue (5): 454-458.doi: 10.13459/j.cnki.cjap.2016.05.018

The expression of calcium sensing receptor(CaSR) and MAPK pathway changes in myocardial cells of epilepsy rats

GUO Jin¹, LIU Yang^1,4, WANG Chao², BAI Ling-ling¹, HAN Xiao-wei², ZHANG Xin-yang¹, SUN Yan-qiu¹, WANG Lu-chuan², JIANG Zhi-mei^1,3

1. The Affiliated the Third Hospital, Jiamusi 154002;
2. The Affiliated the First Hospital Jiamusi University, Jiamusi 154002;
3. Harebin MedicalCollege of Public Health, Harebin 150026, China;
4. Harebin NO.4 Hospital

Received:2015-04-14 Revised:2016-04-19 Online:2016-09-28 Published:2018-06-20
Supported by:
国家自然科学基金资助项目（81300122）；黑龙江省青年科学基金项目（QC2016130）；佳木斯大学校级创新团队（Cxtd201302）

Abstract

Abstract: Objective: To investigate the changes of the myocardial cells in chronic epileptic rat model and to observe the expression of calcium sensing receptor(CaSR) and mitogen-activated proteinkinase(MAPK)pathway changes in epilepsy rats. Methods: The chronic epileptic rat model was induced bypentetrazole (PTZ). Adult male Wistar rats were divided into 5 groups randomly, and there were 12 rats in each group. The rats in model group were treated with a sub-convulsivedose of PTZ (35 mg/kg) by intraperitoneal injection for 28 d. After stopping a week, the same dose of PTZ test was conducted. The control group was treated with isovolumetric saline instead of PTZ by intraperitoneal injection. According to Racine behavior grading standards the rat emerged two levels above epileptic seizure 5 consecutive times, which was considered the chronic epilepsy model successful ignition. The intervention factors included spermine(calcium-sensing receptor agonist, 3 μmol/L) and Chalhex231(calcium-sensing receptor inhibitor, 2 μmol/L). The serum creatine kinase (CK) and creatine kinase isoenzyme(CK-MB)were detected. The cardiac functions, morphological changes of rat myocardial tissue, myocardial cell ultrastructure, myocardial cell calcium sensing receptor and extracellular regulated protein kinase (ERK), p-ERK, p-JNK expression were carried out. Results: Compared with normal control group, CK, CK-MB inPTZ group were increased obviously. The cardiac compliance and left ventricular function were decreased, E/A<1 by echocardiography. The myocardial ultrastructure showed serious injury. The expressions of CaSR and p-JNK were increased, but the expression of p-ERKwas decreased. Spermine could promote the expressions of CaSR and p-JNK, and decrease the expression of p-ERK in epilepsy; however, the role of Chalhex231 wasopposite. Conclusion: The level of CaSR expression increased in chronic epileptic rat model. CaSR activated the expressions of MAPK of the myocardial cells,andthen influenced the cardiac myocyte apoptosis.

Key words: calcium-sensing receptor, mitogen-activated proteinkinase, epilepsy, myocardial cells

GUO Jin, LIU Yang, WANG Chao, BAI Ling-ling, HAN Xiao-wei, ZHANG Xin-yang, SUN Yan-qiu, WANG Lu-chuan, JIANG Zhi-mei. The expression of calcium sensing receptor(CaSR) and MAPK pathway changes in myocardial cells of epilepsy rats[J]. CJAP, 2016, 32(5): 454-458.

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The expression of calcium sensing receptor(CaSR) and MAPK pathway changes in myocardial cells of epilepsy rats

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