The effects of ERK1/2 pathway on the expression of calcium activated chloride channel in hypoxia in PASMCs rat model

doi:10.12047/j.cjap.5448.2017.011

CJAP ›› 2017, Vol. 33 ›› Issue (1): 47-50.doi: 10.12047/j.cjap.5448.2017.011

The effects of ERK1/2 pathway on the expression of calcium activated chloride channel in hypoxia in PASMCs rat model

ZHAO Mei-ping¹, MA Ying-chun³, ZHANG Cong-cong¹, HUANG Lin-jing¹, ZHENG Meng-xiao¹, LI Guan-long⁴, YING Lei^1,2, WANG Wan-tie^1,2

1. Department of Pathophysiology, Wenzhou Medical University, Wenzhou 325035;
2. Research Institute of Wenzhou Medical University in ischemia-reperfusion injury, Wenzhou 325035;
3. Department of Pathology, Lanzhou General Hospital of Lanzhou Military Region, Lanzhou 730050;
4. Zhejiang Provincial Academy of Medical Sciences and Health Research Institute of Toxicology Laboratory, Hangzhou 310013, China

Received:2016-03-11 Revised:2016-10-22 Online:2017-01-28 Published:2018-06-19
Supported by:
浙江省公益性技术应用研究计划项目（2015C37121）

Abstract

Abstract: Objective: To investigate the expression of mRNA and protein of Calcium activated chloride channel (CLCA2) in hypoxic pulmonary artery smooth muscle cell (PASMCs) of rat and it's relationship with ERK1/2 signal pathway.Methods: PASMCs were randomly divided into 5 groups including normal group(N group), hypoxia group(H group), DMSO group(D group), U0126 group (U group) and Staurosporine aglycone group(SA group). The protein expression of CLCA2 in PASMCs was detected by Western blot.The mRNA expression of CLCA2 was detected by half quantitative reverse transcription polymerase chain reaction (RT-PCR).Results: The mRNA and protein expressions of CLCA2 in H group were significantly higher than N group (P<0.01). Comparing with D group,the mRNA and protein expressions of CLCA2 were significantly increased in U group (P<0.01),the mRNA expression of CLCA2 in SA group was obviously decreased (P<0.01) with slightly decreasing of its protein expression.Conclusion: Hypoxia promotes the expressions of mRNA and protein of CLCA2 in rat PASMCs. The ERK1/2 pathway activator Staurosporine aglycone reduces the mRNA and protein expression of CLCA2 in rats PASMCs and the ERK1/2 pathway inhibitor U0126 induces the upregulation of the mRNA and protein expressiosn of CLCA2 in rats PASMCs.

Key words: calcium activated chloride channel, hypoxia, ERK1/2, rat

ZHAO Mei-ping, MA Ying-chun, ZHANG Cong-cong, HUANG Lin-jing, ZHENG Meng-xiao, LI Guan-long, YING Lei, WANG Wan-tie. The effects of ERK1/2 pathway on the expression of calcium activated chloride channel in hypoxia in PASMCs rat model[J]. CJAP, 2017, 33(1): 47-50.

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The effects of ERK1/2 pathway on the expression of calcium activated chloride channel in hypoxia in PASMCs rat model

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