Effect of long noncoding RNA SPRY4-IT1 on proliferation and metastasis of medulloblastoma

doi:10.12047/j.cjap.5448.2017.020

Abstract

Abstract: Objective: To investigate the effects of long non-coding RNA SPRY4-IT1 (LncRNA) on proliferation and metastasis of medul-loblastoma cells.Methods: SPRY4-IT1siRNA and control fluorescence siRNA were transfected into medulloblastoma cell line Daoy with Lipo-fe ctamine 2000 and were divided into control group and si-SPRY4-IT1 group. Relative expression of SPRY4-IT1 mRNA were detected by real-time quantitative PCR. The change of cell proliferation were examined using CCK-8 kit and clone forming experiment. The change of cell inva-sion and metastasis were examined by matrigel invasion assay and cell metastasis experiment respectively, The expression of matrix metallopro-teinase MMP-2 and MMP-9 were detected by Western blot.Results: In si-SPRY4-IT1 group,the SPRY4-IT1 mRNA expression level, cell pro-liferation in vitro,cell invasion and migration ability, MMP-2 protein expression were significantly lower than those in the control group.Conclusion: Interference SPRY4-IT1 expression has prominent inhibitory effect on the cell proliferation、invasion and metastasis of medulloblastoma cell line Daoy.

Key words: medulloblastoma, long noncoding RNA (LncRNA), SPRY4-IT1, proliferation, invasion and metastasis

SHI Peng-fei, JI Hai-long, LUO Yong-kang, MAO Tian-ming, CHEN Xi, ZHOU Kai-yu. Effect of long noncoding RNA SPRY4-IT1 on proliferation and metastasis of medulloblastoma[J]. CJAP, 2017, 33(1): 78-82.

References

[1] Mueller S, Chang S. Pediatric brain tumors:current treat-ment strategies and future therapeutic approaches[J]. Neu-rotherapeutics, 2009, 6(3):570-586.
[2] Gupta T, Jalali R, Goswami S, et al. Early clinical out-comes demonstrate preserved cognitive function in children with average risk medulloblastoma when treated with hyper-fr actionated radiation therapy[J]. Int J Radiat Oncol Biol Phys, 2012, 83(5):1534-1540.
[3] Neglia JP, Robison LL, Stovall M, et al. New primary neo-plasms of the central nervous system in survivors of childhood cancer:a report from the Childhood Cancer Survivor Study[J]. J Natl Cancer Inst, 2006, 98(21):1528-1537.
[4] Giangaspero F, Wellek S, Masuoka J, et al. Stratification of medulloblastoma on the basis of histopathological grading[J]. Acta Neuropathol, 2006, 112(1):5-12.
[5] Ma L, Bajic VB, Zhang Z. On the classification of long non-coding RNAs[J]. RNA Biol, 2013, 10(6):925-933.
[6] Liu H, Lv Z, Guo E. Knockdown of long noncoding RNA SPRY4-IT1 suppresses glioma cell proliferation, metastasis and epithelial-mesenchymal transition[J]. Int J Clin Exp Pathol, 2015, 8(8):9140-9146.
[7] Djebali S, Davis CA, Merkel A, et al. Landscape of tran-scription in human cells[J]. Nature, 2012, 489(7414):101-108.
[8] Spizzo R, Almeida MI, Colombatti A, et al. Long non-cod-ing RNAs and cancer:a new frontier of translational research[J]. Oncogene, 2012, 31(43):4577-4587.
[9] Mercer TR, Dinger ME, Mattick JS. Long non-coding RNAs:insights into functions[J]. Nature Reviews Genetics, 2009, 10(3):155-159.
[10] Gupta RA, Shah N, Wang KC, et al. Long non-coding RNA HOTAIR reprograms chmmatin state to promote cancer metastasis[J]. Nature, 2010, 464(7291):1071-1076.
[11] Zhang HM, Yang FQ, Yan Y, et al. High expression of long non-coding RNA SPRY4-IT1 predicts poor prognosis of clear cell renal cell carcinoma[J]. Int J Clin Exp Pathol, 2014, 7(9):5801-5809.
[12] Ota T, Suzuki Y, Nishikawa T, et al. Complete sequencing and characterization of 21, 243 full-length human cDNAs[J]. Nat Genet, 2004, 36(1):40-45.
[13] Khaitan D, Dinger ME, Mazaret J, et al. The melanoma-upregulated long noncoding RNA SPRY4-IT1 modulates apop-tosis and invasion[J]. Cancer Res, 2011, 71(11):3852-3862.
[14] Mazar J, Zhao W, Khalil AM, et al. The functional charac-terization of long noncoding RNA SPRY4-IT1 in human melanoma cells[J]. Oncotarget, 2014, 5(19):8959-8969.
[15] 聂鸿靖, 田云梅, 张东祥, 等. 高原低氧习服大鼠红细胞变形性的变化规律及其分子机制[J]. 中国应用生理学杂志, 2011, 27(1):23-28.
[16] Peng W, Wu G, Fan H, et al. Noncoding RNA SPRY4-IT1 predicts poor patient prognosis and promotes tumorigenesis in gastric cancer[J]. Tumor Biol, 2015, 36(9):6751-6758.
[17] Xie M, Nie FQ, Sun M, et al. Decreased long noncoding RNA SPRY4-IT1 contributing to gastric cancer cell metastasis partly via affecting epithelial mesenchymal transition[J]. J Transl Med, 2015, 13:250.