Effects and mechanism of bFGF on rat myoblast oxidative injury induced by hydrogen peroxide

doi:10.12047/j.cjap.5446.2017.040

Abstract

Abstract: Objective: To explore the protective role of basic fibroblast growth factor (bFGF) on attenuating hydrogen peroxide-induced injury in cultured rat myoblasts. Methods: Cultured rat myoblasts at growth phase were randomly divided into four groups (n=6):control group (control), bFGF group (bFGF), model group(H₂O₂) and the treatment group (bFGF + H₂O₂). Model group was treated with 100 μmol/L hydrogen peroxide for 4h. B-cell lymphoma-2 (Bcl-2) positive particles were detected by immunohistochemistry; Reactive oxygen species (ROS) and expression for Bcl-2 associated X protein (Bax), Bcl-2 and Cytochrome C (Cyt. C) fluorescence were observed under the invented microscope; Cyt. C and Poly ADP-ribose polymerase(PARP)protein were assessed by Western blot. Results: Compared with control group, the myoblats in the model group showed low expression of Bcl-2 positive particles, accompanied by high expression of ROS level and Cyt. C fuorescence (P < 0.05); Compared with model group, bFGF enhanced Bcl-2 activity of the myoblasts, and significantly downregulated Cyt. C and PARP expression (P < 0.05). Conclusion: bFGF could attenuate oxidative injury of rat myoblasts induced by hydrogen peroxide, which mechanism might be related to enhanced Bcl-2 and reduced ROS, Cyt. C levels.

Key words: pressure ucler, deep tissue injury, oxidative stress, rat myoblasts

MAO Ting-ting, FANG Hong-bo, WANG Xiao-hui, PAN Ying-ying, XIE Hao-huang, ZHANG Hong-yu, XIAO Jian, JIANG Li-ping. Effects and mechanism of bFGF on rat myoblast oxidative injury induced by hydrogen peroxide[J]. CJAP, 2017, 33(2): 159-163.

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