Effects of the maca extract on the ultrastructures of mitochondria in the spinal nerve cell and exercise endurance

doi:10.12047/j.cjap.5601.2017.127

CJAP ›› 2017, Vol. 33 ›› Issue (6): 535-538.doi: 10.12047/j.cjap.5601.2017.127

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Effects of the maca extract on the ultrastructures of mitochondria in the spinal nerve cell and exercise endurance

YU Fa-rong¹, YANG Bo¹, LI Zuo-ping², LIAN Xiu-zhen¹, XIE Ming-ren¹, LI Deng-lou¹, ZHANG Shi-shuang¹

1. Key Laboratory of Evidence of Science and Technology Research and Application, Gansu Institute of Political Science and Law, Lanzhou 730070, China;
2. Department of Physical Education, Gansu Institute of Political Science and Law, Lanzhou 730070, China

Received:2016-07-28 Revised:2017-05-15 Online:2017-11-28 Published:2018-06-19
Contact: 李作平,Tel:18189651683;E-mail:tim9898@163.com E-mail:tim9898@163.com
Supported by:
甘肃省基础研究创新群体项目（145RJIA333）；兰州市科技计划项目（2014-1-182；2015-3-80）；甘肃省高校科技创新团队项目（2016C-09）；兰州市人才创新创业项目（2016-RC-85）；西北民族地区侦查理论与实务研究中心项目；甘肃政法学院重大项目（2016XZD15）；甘肃政法学院"文翰学者"项目

Abstract

Abstract: Objective: To investigate the effects of maca extract on the ultrastructures of mitochondria in the spinal nerve cell and exercise endurance.Methods: The Wistar rats were randomly divided into 5 groups, including the control group (no swimming), the swimming group (free swimming), and 3 treatment groups treated with the maca extract at the doses of 4.0, 5.3 and 8.0 g/kg body weight. The animals in swimming and treatment groups were then for free swimming in the circulating water flow daily for 15 days. On the 16^th day after swimming endurance, the spinal and muscular tissues were collected from all groups. The mitochondrial ultrastructures of the neurons of the spinal cells were observed with the projection electron microscope, and the levels of the glycogen, malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and Ca²⁺ in muscle tissues were determined by the RIA method.Results: When rats were treated with maca extract (at 4.0, 5.3, 8.0 g/kg body weight), the total swimming time and the swimming duration before sinking were increased by 19.83%, 60.28%, 77.55%, and 55.34%, 73.91%, 94.47%, respectively, compared with the simple swimming group(P<0.01), while the sinking times were decreased by 34.35%, 51.18% and 57.96%, compared with those of the swimming group. Also, the levels of SOD, GSH-Px, and muscle glycogen in three treatment groups were enhanced by 5.12%, 22.74%, 52.53%, 44.22%, 77.79%, 98.45%(P<0.01), and 35.08%, 47.83%,81.88% (P<0.01)respectively over the swimming rats without treatment, but the MDA content and the Ca²⁺ levels were reduced by 20.10%, 31.49% 38.72%, and 6.42%, 17.58%, 26.35%,compared with the simple swimming group(P<0.01). In addition, compared to the swimming group, the mitochondrial densities of volume (VD), surface (SD) and numbers (ND) of spinal nerve cells in rats treated with maca extract (4.0, 5.3, 8.0 g/kg body weight) were reduced by 7.79%, 18.18%, 31.17%, 16.95%, 27.34%, 43.31% and 13.51%, 23.19%, 43.15%, respectively.Conclusion: Our results demonstrated the protective effects of maca extract on the mitochondria of spinal cell and suggested that maca extract could improve the muscle antioxidant activity by increasing the levels of SOD, GSH-Px, and muscle glycogen.

Key words: maca extract, movement endurance, mitochondria, Wistar rats

YU Fa-rong, YANG Bo, LI Zuo-ping, LIAN Xiu-zhen, XIE Ming-ren, LI Deng-lou, ZHANG Shi-shuang. Effects of the maca extract on the ultrastructures of mitochondria in the spinal nerve cell and exercise endurance[J]. CJAP, 2017, 33(6): 535-538.

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Effects of the maca extract on the ultrastructures of mitochondria in the spinal nerve cell and exercise endurance

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