Effects of aerobic exercise combined with chlorella pyrenoidos of disintegrated cell wall on some indicators of lipid metabolism in rats with high-fat diet

doi:10.12047/j.cjap.5688.2018.101

Abstract

Abstract: Objective: To study the effects of aerobic exercise combined with chlorella pyrenoidos of disintegrated cell wall on the lipid metabolism in rats with high-fat diet. Methods: Fifty-five male Wistar rats were subjected to adaptive feeding for 4 days and weight-free swimming training for 3 days, 20 min/d. After eliminating 5 rats that were not suitable for swimming training, the other rats were randomly divided into 5 groups according to their body weight:control group (C group), high fat diet group (H group), high-fat diet + chlorella group(HC group), high fat diet + aerobic exercise group (HM group), high fat diet + chlorella + aerobic exercise group (HMC group), 10 in each group. The HM and HMC group were subjected to 60 min/d swimming training for 6 weeks with non-weight-bearing. Group C were fed regular diet. The other groups were fed with high-fat diet, the rats in group HC and HMC were intragastrically treated with chlorella pyrenoidos of disintegrated cell wall at the dose of 3.9 g/(kg·d), the volume was 5 ml/kg, and the other groups are given equivalent saline. The Lee's index and biochemical indexes of blood and liver were measured after 6 weeks. Results: Compared with group C, Lee's index, serum levels of free fatty acids(FFA), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-c), liver FFA and interleukin-10 (IL-10) were increased significantly (P<0.01), the serum level of high-density lipoprotein cholesterol (HDL-c) was decreased significantly (P<0.01) in group H. Compared with group H, Lee's index, serum FFA, IL-6, TNF-α, TC, TG, LDL-c, liver FFA and IL-10 were decreased significantly (P<0.05 or P<0.01), serum level of HDL-c was increased significantly (P<0.05 or P<0.01) in group HC, HM and HMC. Compared with group HC and HM, Lee's index, serum FFA, IL-6, TNF-α, TC, TG, LDL-c, liver FFA and IL-10 were decreased significantly (P<0.05), serum level of HDL-c was increased significantly (P<0.05) in group HMC. Conclusion: Aerobic exercise and chlorella pyrenoidos of disintegrated cell wall can improve lipid metabolism in rats with high-fat diet and reduce the lipid toxicity caused by obesity. Joint intervention is more effective than single intervention.

Key words: aerobic exercise, chlorella pyrenoidos of disintegrated cell wall, high-fat diet, rat, lipid metabolism

ZHANG Jing, MIAO Gang, CAO Jian-min, ZHOU Hai-tao, NIU Yan-long, ZHANG Yue, REN Yi, BAO Xin-yue, XING Ya-wen. Effects of aerobic exercise combined with chlorella pyrenoidos of disintegrated cell wall on some indicators of lipid metabolism in rats with high-fat diet[J]. CJAP, 2018, 34(5): 445-449.

References

[1] Maury E, Brichard SM.Adipokine dysregulation, adipose tissue inflammation and metabolic syndrome[J]. Mol Cell Endocrinol, 2010, 314(1):1-16.
[2] Kralovec JA, Metera KL, Kumara JR, et al. Immunostimulatory principles from Chlorella pyrenoidosa-part1:Isolation and biological assessment in vitro[J]. Phytomedicine, 2007, 14(1):57-64.
[3] 付德荣, 孙小华, 刘承宜, 等. 有氧运动加谷氨酰胺补充对2型糖尿病大鼠骨骼肌炎症因子NF-κB、MPO及MCP-1基因表达的影响[J]. 体育科学, 2012, 32(12):55-61.
[4] 晏春根. 非脂肪组织脂质毒性及其机制[J]. 实用医学杂志, 2005, 21(19):2214-2215.
[5] 刘宇, 韩延柏, 任宝利. 营养、运动与代谢综合征[J]. 沈阳体育学院学报, 2009, 28(6):85-87.
[6] 林亚秋. 肿瘤坏死因子-α对大鼠脂肪细胞凋亡的影响. 咸阳:西北农业科技大学, 2004.
[7] Scheller J, Chalaris A, Schmidt-Arras D, et al. The pro- and anti-inflammatory properties of the cytokine interleukin-6[J]. Biochim Biophys Acta, 2011, 1813(5):878-888.
[8] 何明, 涂长春, 黄起壬, 等. Lee's指数用于评价成年大鼠肥胖程度的探讨[J]. 中国临床药理学与治疗学杂志, 1997, 2(3):177-179.
[9] 钱万万. 高脂膳食对大鼠心肌的脂毒性作用及有氧运动、原花青素干预效应的实验研究. 苏州:苏州大学, 2015.
[10] 韩士群, 张振华, 刘海琴. 小球藻生长因子的生理功能研究[J]. 江苏农业科学, 2003, 31(5):99-101.
[11] 王炳岩, 季宇彬. 海藻多糖对白血病L615小鼠LPO含量及GR、GSH-Px、CAT、SOD酶活性的影响[J]. 中医药信息, 1994, 11(5):43-45.
[12] Vijayavel K, Anbuselvam C, Balasubramanian MP. Antioxidant effect of the marine algae Chlorella vulgaris against naphthalene-induced oxidative stress in the albino rats[J]. Mol Cell Biochem, 2007, 303(1-2):39-44.
[13] 封会茹, 计融, 李燕俊, 等. 破壁蛋白核小球藻对小鼠免疫调节作用[J]. 中国公共卫生, 2008, 24(7):836-837.
[14] 愈建江, 李荷芳, 周汉秋. 10种海洋微藻总脂中性脂极性脂的脂肪酸组成[J]. 水生生物学报, 1999, 23(5):481-488.
[15] 鲁林, 刘桂, 王晓慧, 等. 有氧运动同时补充玉米肽对肥胖大鼠脂肪分解关键酶的影响[J]. 中国应用生理学杂志, 2016, 32(4):326-331.
[16] 王小梅, 景会锋. 有氧运动对高脂饮食大鼠血液中胃促生长素、血糖及血脂的影响[J]. 中国应用生理学杂志, 2014, 30(1):53-59.