The effects of anthocyanin on chronic inflammatory pain induced by complete Freund's adjuvant and its mechanism

doi:10.12047/j.cjap.5671.2018.106

CJAP ›› 2018, Vol. 34 ›› Issue (5): 476-480.doi: 10.12047/j.cjap.5671.2018.106

The effects of anthocyanin on chronic inflammatory pain induced by complete Freund's adjuvant and its mechanism

WANG Jing¹, CHEN Ze-feng², HE Chun-xia², WANG Li-li², ZHENG Jia-hui², ZHANG Hao³, WANG Zhan-lei⁴, LU Ya-ping⁵

1. Department of Physiology, School of Basic Medical Sciences, Wannan Medical College, Wuhu 241002;
2. 2013 level clinical specialty, Wannan Medical College, Wuhu 241002;
3. 2015 level clinical specialty, Wannan Medical College, Wuhu 241002;
4. 2014 level anaesthetics specialty, Wannan Medical College, Wuhu 241002;
5. College of Life Science, Anhui Normal University, Wuhu 241002, China

Received:2018-01-12 Revised:2018-08-20 Online:2018-09-28 Published:2019-02-21
Supported by:
国家自然科学基金面上项目（81671586）；安徽省教育厅高校优秀人才计划项目（gxyq2017036）；皖南医学院重点科研培育基金（WK2016Z10）；皖南医学院中青年基金（WK201507）；安徽省大学生创新创业训练项目（201710368116，201610368065，201510368064）

Abstract

Abstract: Objective: To investigate the effects and mechanisms of anthocyanin from Ligustrum vicaryi on chronic inflammatory pain induced by complete Freund's adjuvant. Methods: Thirty male SD rats were randomly divided into three groups (n=10):normal saline control group (NS), chronic inflammatory pain model group(Mod, injected with complete Freund's adjuvant(CFA) 100 μl to the left hind leg), anthocyanin treatment group(Ant, dosed with anthocyanins (90 m), mechanical pain threshold (MPT), and left toe volume in each group were measured before modeling and 1,3,5,7,9,11,13 days after operation. Antioxidant indexes in serum were mensurated by spectrophotometer, and the total capsaicin receptor (TRPV1) and phosphorylated capsaicin receptor (p-TRPV1) in hippocampus were detected by Western blot. Results: In comparison with controls, HPT and MPT were improved (P<0.05),toe swelling was reduced(P<0.05), the serum level of SOD was increased (P<0.01), while the levels of MDA and NO were decreased (P<0.05), the ratio of P-TRPV1/TRPV1 protein was depressed in Mod rat hippocampal region treated with anthocyanin. Conclusion: The results show that anthocyanins has an analgesic effect on chronic inflammatory pain induced by CFA, and its mechanism may be related to the improvement of antioxidant capacity and the reduction of TRPV1 phosphorylation.

Key words: anthocyanins, chronic inflammatory pain, pain threshold, TRPV1

WANG Jing, CHEN Ze-feng, HE Chun-xia, WANG Li-li, ZHENG Jia-hui, ZHANG Hao, WANG Zhan-lei, LU Ya-ping. The effects of anthocyanin on chronic inflammatory pain induced by complete Freund's adjuvant and its mechanism[J]. CJAP, 2018, 34(5): 476-480.

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The effects of anthocyanin on chronic inflammatory pain induced by complete Freund's adjuvant and its mechanism

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