The protective effects  of lycium barbarum polysaccharides on retinal  neurons in diabetic rats and its mechanism

doi:10.12047/j.cjap.5706.2019.014

Abstract

Abstract: Objective:To clarify whether lycium barbarum polysaccharides (LBP) have protective effects on retina neuronal cells in diabetic rats and to identify the related mechanism involved in this process. Methods: Eighteen SD rats were randomly divided into 3 groups ( n= 6): normal control group (NC), diabetes mellitus group (DM) and LBP-treatment group (DM+LBP). The diabetic rat model was induced by single intraperitoneal injection of streptozotocin (STZ). The rats in DM+LBP group were treated with LBP at the dose of 1 mg/kg by gavage, once a day for 12 weeks. After the treatment, the weight and blood glucose, the generation of reactive oxygen species (ROS), the surviving retinal ganglion cells (RGCs) and amacrine cells and the protein expressions of nuclear factor E2-related factor 2 (Nrf2) and the heme oxygenase-1 (HO-1) were detected. Results: The successful rate of diabetic model was 100%. Compared with NC group, the rats of DM group caused weight loss, elevated blood glucose, a marked increase of ROS generation and a significant decrease in the number of RGCs and amacrine cells (P＜0.01), and these effects were diminished or abolished by LBP treatment. Meanwhile, LBP significantly increased the expressions of Nrf2 and HO-1 in the retinas of diabetic rats (P＜0.01). Conclusion: LBP can improve retinal oxidative stress and exert beneficial neuroprotective effects in diabetic rats, and its mechanism may be associated with the activation of the Nrf2/HO-1 antioxidant pathway.

Key words: lycium barbarum polysaccharides, rat, diabetic retinopathy, oxidative stress, nuclear factor E2-related factor 2, heme oxygenase-1

PAN Hong, SHI Zhen, YANG Tai-guo, YU La-mei, XU Ai-li. The protective effects of lycium barbarum polysaccharides on retinal neurons in diabetic rats and its mechanism[J]. CJAP, 2019, 35(1): 55-59.

References

[1] McAuley AK, Sanfilippo PG, Hewitt AW, et al. Vitreous biomarkers in diabetic retinopathy: a systematic review and meta-analysis[J]. J Diabetes Complications, 2014, 28(3): 419-425.
[2] Neelam K, Goenadi CJ, Lun K, et al. Putative protective role of lutein and zeaxanthin in diabetic retinopathy[J]. Br J Ophthalmol, 2017, 101(5): 551-558.
[3] Li J, Wang JJ, Yu Q, et al. Inhibition of reactive oxygen species by Lovastatin downregulates vascular endothelial growth factor expression and ameliorates blood-retinal barrier breakdown in db/db mice: role of NADPH oxidase 4[J]. Diabetes, 2010, 59(6): 1528-1538.
[4] Liu Q, Zhang F, Zhang X, et al. Fenofibrate ameliorates diabetic retinopathy by modulating Nrf2 signaling and NLRP3 inflammasome activation[J]. Mol Cell Biochem, 2018, 445(1-2): 105-115.
[5] He M, Pan H, Chang RC, et al. Activation of the Nrf2/HO-1 antioxidant pathway contributes to the protective effects of Lycium barbarum polysaccharides in the rodent retina after ischemia-reperfusion-induced damage[J]. PLoS One, 2014, 9(1): e84800.
[6] 马雅玲, 陈凤, 李小璐. 枸杞多糖对糖尿病大鼠视网膜保护作用的研究[J]. 宁夏医科大学学报, 2013, 35(8): 837-840.
[7] Li XM, Ma YL, Liu XJ.Effect of the Lycium barbarum polysaccharides on age-related oxidative stress in aged mice[J]. J Ethnopharmacol, 2007, 111(3): 504-511.
[8] Li XM. Protective effect of Lycium barbarum polysaccharides on streptozotocin-induced oxidative stress in rats[J]. Int J Biol Macromol, 2007, 40(5): 461-465.
[9] Shan X, Zhou J, Ma T, et al. Lycium barbarum polysaccharides reduce exercise-induced oxidative stress[J]. Int J Mol Sci, 2011, 12(2): 1081-1088.
[10]许拓, 凌宏艳, 龙佳, 等. 枸杞多糖对HepG2细胞胰岛素抵抗的改善作用及机制研究[J]. 中国应用生理学杂志, 2017, 33(6): 568-571.
[11]Brownlee M. The pathobiology of diabetic complications: a unifying mechanism[J]. Diabetes, 2005, 54(6): 1615-1625.
[12]宋丽君. 南瓜多糖对糖尿病大鼠血糖、血脂及氧化应激能力的影响[J]. 中国应用生理学杂志, 2015, 31(1): 65-66.
[13]潘虹, 蒋淑君, 刘红霞, 等. 莱菔硫烷对糖尿病大鼠视网膜神经细胞的保护作用[J]. 中国病理生理杂志, 2017, 33(11): 1938-1944.
[14]Masuda T, Shimazawa M, Hara H. Retinal diseases associated with oxidative stress and the effects of a free radical scavenger (edaravone)[J]. Oxid Med Cell Longev, 2017, 2017: 9208489.
[15]Nabavi SF, Barber AJ, Spagnuolo C, et al. Nrf2 as molecular target for polyphenols: A novel therapeutic strategy in diabetic retinopathy[J]. Crit Rev Clin Lab Sci, 2016, 53(5): 293-312.
[16]Fan J, Xu G, Jiang T, et al. Pharmacologic induction of heme oxygenase-1 plays a protective role in diabetic retinopathy in rats[J]. Invest Ophthalmol Vis Sci, 2012, 53(10): 6541-6556.

The protective effects of lycium barbarum polysaccharides on retinal neurons in diabetic rats and its mechanism

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	HU Jing, LI Fa-dong, SHAO Xiao-ting, LI Si-wei, ZHANG Xin-ying, ZHAO Bing-bing, XU Chang-qing, WEI Can. Spermine attenuates high glucose-induced myocardial fibrosis by regulating the cell cycle [J]. CJAP, 2020, 36(3): 193-196.
[2]	ZHU Jian-zhong, ZHAO Can, SUI Yue-lin, LIU Yuan-yuan, QIAO Yue-bing. Effects of leptin on lipid metabolism and inflammatory factors in diabetic rats [J]. CJAP, 2020, 36(3): 197-201.
[3]	SUN Xiao-juan, FENG Wu-long, HOU Na, LI Na, JIANG Wei-li. Effects of aerobic exercise and resveratrol on the expressions of JAK2 and TGF-β1 in renal tissue of type 2 diabetes rats [J]. CJAP, 2020, 36(3): 202-206.
[4]	LIU Kai-li, DU Xin-xin, ZHANG Wen-qin, WU Ya-li, WANG Xi, WANG Chun-fang, CUI Xiang-li. Overexpression of miR-31 regulates TLR4/NF-κB signaling pathway and apoptotic protein in colitis model mice [J]. CJAP, 2020, 36(3): 211-215.
[5]	SHI Hui, LIANG Xiao-shan, HUANG Li-wen, LUO Zhi-gang, TAN Long. The optimization and assessment of the method for inducing hyperuricemia in rats [J]. CJAP, 2020, 36(3): 223-227.
[6]	HAN Jie, LIU Zi-yi, FANG Zhen, TIAN Lei, YANG Dan-feng, XI Zhu-ge, LIU Xiao-hua. Effects of repeated immobilization stress on hypothalamic-pituitary- ovarian axis in female rats [J]. CJAP, 2020, 36(3): 245-249.
[7]	CHEN Yu-feng, DONG Fan-he, LOU Yun-wei, SHOU Jin-hao, ZHANG Hui-ting, ZHOU Yi-chao, YAN Ming, MAO Hong-jiao, ZHANG Yun. Effect of psoralen on rat osteoblasts injuries induced by TCP wear particles in vitro and its mechanism [J]. CJAP, 2020, 36(3): 255-260.
[8]	. [J]. CJAP, 2020, 36(3): 265-267.
[9]	XU Chang-shun, SUN Pei, LIN Chun. A new design puncture needle and a device of microcatheter protection for lumbar intrathecal catheterization in rats [J]. CJAP, 2020, 36(3): 283-288.
[10]	WANG Ling-xing, HONG Shan-yan, YANG Mei-li, HUANG Hong-hong. Effects of butylphthalide on microglia activation in frontal lobe of rats after chronic sleep deprivation [J]. CJAP, 2020, 36(2): 106-110.
[11]	WAN Jian-yong, ZHANG Yong, LIU Shi-qiang, HUA Tian-miao, SUN Qing-yan. Effects of exercise intervention on BIM signaling in liver of insulin resistance rats [J]. CJAP, 2020, 36(2): 115-118.
[12]	JIN Zhao, CAO Zheng-tao, WANG Hai-xia, ZHANG Li-hui, LI Yi-feng, ZHOU Yu-bin, WANG Quan, LI Bao-hui, XU Yan, WEI Xiao-yang, WANG Hong, LIN Rong, YANG JING-hui, GENG Xi-chen. Changes of regional cerebral oxygen saturation under gradual-onset rate +Gz exposure [J]. CJAP, 2020, 36(2): 130-133.
[13]	ZHOU Bi-ye, LI Jing, LI Ming-gao, SUN Hai-wen, ZHOU Jia-xing, HAN Lei. Effect of repeated horizontal acceleration exposure on cardiac structure in New Zealand rabbits [J]. CJAP, 2020, 36(2): 134-137.
[14]	YAO Liang-xue, HAN Jia-xin, WEI Pei-yun, HOU Si-bo, HE Xiao-jian, ZHANG Rui-ling, DU Ai-lin. Effects of butylphthalide on H₂S/CBS pathway in hippocampal and amygdala and learning and memory ability in chronic alcoholism rats [J]. CJAP, 2020, 36(2): 138-142.
[15]	LIANG Wan-ling, XIAO Tian-bao, YUAN Feng, CAO Yi-bo, YAN Deng-guo. Effects of microRNA95 targeting FOXD1 gene on radiosensitivity of colorectal cancer loVo cells and its mechanism [J]. CJAP, 2020, 36(2): 148-151.