Effects of Shenmai injection on the expression of p38MAPK and the  apoptosis-related genes in lung injury induced by intestinal  ischemia/reperfusion in rats

doi:10.12047/j.cjap.5713.2019.016

Abstract

Abstract: Objective:To observe the effects of Shenmai injection(SM) on p38MAPK and the apoptosis-related genes in lung injury induced by intestinal ischemia reperfusion (I/R) in rats and to investigate the protective mechanism of SM. Methods: Rat model of intestinal I/R injury was established with clamping of the superior mesenteric artery (SMA) for 60 min and then clamping was relieved for 60 min. Twenty-four SD rats were randomly divided into three groups with eight rats in each: control group, intestinal ischemia/reperfusion group(I/R group), Shenmai injection treated group (SM+I/R group). Lung wet/dry weight ratio(W/D), the contents of phosphatidylcholine (PC) and total phospholipid(TPL) which are the major ingredients of pulmonary surfactant were measured, as well as the expression levels of p38MAPK, Bcl-2 and Bax proteins in lung tissue were examined by using immunohistochemical method. Results: Compared with control group, lung W/D was significantly increased, the contents of PC and TPL were significantly decreased, the protein expression levels of p38MAPK, Bcl-2 and Bax were significantly increased in I/R group (all P＜0.01). But Bax protein expression was much greater than Bcl-2 protein expression, the ratio of Bcl-2 to Bax were significantly decreased in I/R group than that in control group (P＜0.01). Compared with I/R group, lung W/D was significantly decreased, while the contents of PC and TPL were significantly increased, the p38MAPK and Bax protein expression levels were significantly decreased in SM+I/R group (all P＜0.01); both Bcl-2 protein expression and the ratio of Bcl-2 to Bax were significantly increased in SM+I/R group than those in I/R group (P＜0.01). The correlation analysis indicated that the expression level of p38MAPK protein in lung tissue was negatively correlated with the contents of PC and the ratio of Bcl-2 to Bax (r is -0.787 and -0.731, all P＜0.01). Conclusion: SM can protect the lung injury induced by intestinal I/R injury, which may be mediated by inhibiting the activation of p38MAPK, improving the ratio of Bcl-2 to Bax to inhibit lung apoptosis.

Key words: Shenmai injection, intestine, ischemia/reperfusion, acute lung injury, p38MAPK, apoptosis, rat

ZHAO Jia-han, JIA Yu-han, TANG Ya-ting, LIN Yi-xin, WANG Yan-lei. Effects of Shenmai injection on the expression of p38MAPK and the apoptosis-related genes in lung injury induced by intestinal ischemia/reperfusion in rats[J]. CJAP, 2019, 35(1): 65-68.

References

[1] Jia Z, Lian W, Shi H, et al. Ischemic postconditioning protects against intestinal ischemia/reperfusion injury via the HIF-1α/miR-21 axis [J]. Sci Rep, 2017, 7(1): 16190.
[2] Onder A, Kapan M, Gümüs M, et al. The protective effects of curcumin on intestine and remote organs against mesenteric ischemia/reperfusion injury [J]. Turk J Gastroenterol, 2012, 23(2): 141-147.
[3] Tan H, Chen L, Ma J. Penehyclidine hydrochloride post-conditioning reduces ischemia/reperfusion-induced cardiomyocyte apoptosis in rats [J]. Exp Ther Med, 2017, 14(5): 4272-4278.
[4] Yu Y, Liu Q, Guo S, et al. 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin promotes endothelial cell apoptosis through activation of EP3/p38MAPK/Bcl-2 pathway[J]. J Cell Mol Med, 2017, 21(12): 3540-3551.
[5] 黄林静, 张聪聪, 赵美平, 等. MAPK信号通路对大鼠低氧性PASMCs增殖、凋亡的调控[J]. 中国应用生理学杂志, 2017, 33(3): 226-230.
[6] 王艳蕾, 崔国金, 蔡庆艳, 等. 参麦注射液对肠缺血再灌注肺脂质过氧化损伤的影响[J]. 天津医药, 2010, 38(11): 986-989.
[7] 高晓明, 宋建良, 谢翠微, 等. 心钠素与实验性肺水肿[J]. 北京医科大学学报, 1987, 19(2): 69-71.
[8] 李影林. 中华医学检验全书[M]. 北京: 人民卫生出版社, 1996: 737-739.
[9] Zhao L, Gu Q, Xiang L, et al. Curcumin inhibits apoptosis by modulating Bax/Bcl-2 expression and alleviates oxidative stress in testes of streptozotocin-induced diabetic rats [J]. Ther Clin Risk Manag, 2017, 13: 1099-1105.
[10]Cao S, Zhu X, Du L. P38 MAP kinase is involved in oleuropein-induced apoptosis in A549 cells by a mitochondrial apoptotic cascade [J]. Biomed Pharmacother, 2017, 95: 1425-1435.
[11]Zhao HG, Zhou SL, Lin YY, et al. Toxicarioside N induces apoptosis in human gastric cancer SGC-7901 cell by activating the p38MAPK pathway [J]. Arch Pharm Res, 2018, 41(1): 71-78.
[12]Cuenda A, Rousseau S. p38 MAP-kinases pathway regulation, function and role in human diseases [J]. Biochim Biophys Acta, 2007, 1773(8): 1358-1375.
[13]Corre I, Paris F, Huot J. The p38 pathway, a major pleiotropic cascade that transduces stress and metastatic signals in endothelial cells [J]. Oncotarget, 2017, 8(33): 55684-55714.
[14]Ye LF, Zheng YR, Wang LH. Effects of Shenmai injection and its bioactive components following ischemia/reperfusion in cardiomyocytes [J]. Exp Ther Med, 2015, 10(4): 1348-1354.
[15]于齐宏, 杜奕奇, 李兆申, 等. 参麦注射液减轻大鼠重症急性胰腺炎相关性肺损伤[J]. 第二军医大学学报, 2013, 34(11): 1183-1186.
[16]刘小芳. 参麦注射液治疗急性肺损伤的疗效及对血清ICAM-1、ET-1及NO的影响[J]. 现代中西医结合杂志, 2017, 26(25): 2784-2786.
[17]王艳蕾, 景友玲, 赵景霞, 等. 参麦注射液对肢体缺血/再灌注时肺脂质过氧化损伤的防护作用[J]. 中国应用生理学杂志, 2006, 22(1): 13-15.

Effects of Shenmai injection on the expression of p38MAPK and the apoptosis-related genes in lung injury induced by intestinal ischemia/reperfusion in rats

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	HU Jing, LI Fa-dong, SHAO Xiao-ting, LI Si-wei, ZHANG Xin-ying, ZHAO Bing-bing, XU Chang-qing, WEI Can. Spermine attenuates high glucose-induced myocardial fibrosis by regulating the cell cycle [J]. CJAP, 2020, 36(3): 193-196.
[2]	ZHU Jian-zhong, ZHAO Can, SUI Yue-lin, LIU Yuan-yuan, QIAO Yue-bing. Effects of leptin on lipid metabolism and inflammatory factors in diabetic rats [J]. CJAP, 2020, 36(3): 197-201.
[3]	SUN Xiao-juan, FENG Wu-long, HOU Na, LI Na, JIANG Wei-li. Effects of aerobic exercise and resveratrol on the expressions of JAK2 and TGF-β1 in renal tissue of type 2 diabetes rats [J]. CJAP, 2020, 36(3): 202-206.
[4]	LIU Kai-li, DU Xin-xin, ZHANG Wen-qin, WU Ya-li, WANG Xi, WANG Chun-fang, CUI Xiang-li. Overexpression of miR-31 regulates TLR4/NF-κB signaling pathway and apoptotic protein in colitis model mice [J]. CJAP, 2020, 36(3): 211-215.
[5]	SHI Hui, LIANG Xiao-shan, HUANG Li-wen, LUO Zhi-gang, TAN Long. The optimization and assessment of the method for inducing hyperuricemia in rats [J]. CJAP, 2020, 36(3): 223-227.
[6]	HAN Jie, LIU Zi-yi, FANG Zhen, TIAN Lei, YANG Dan-feng, XI Zhu-ge, LIU Xiao-hua. Effects of repeated immobilization stress on hypothalamic-pituitary- ovarian axis in female rats [J]. CJAP, 2020, 36(3): 245-249.
[7]	CHEN Yu-feng, DONG Fan-he, LOU Yun-wei, SHOU Jin-hao, ZHANG Hui-ting, ZHOU Yi-chao, YAN Ming, MAO Hong-jiao, ZHANG Yun. Effect of psoralen on rat osteoblasts injuries induced by TCP wear particles in vitro and its mechanism [J]. CJAP, 2020, 36(3): 255-260.
[8]	. [J]. CJAP, 2020, 36(3): 265-267.
[9]	XU Chang-shun, SUN Pei, LIN Chun. A new design puncture needle and a device of microcatheter protection for lumbar intrathecal catheterization in rats [J]. CJAP, 2020, 36(3): 283-288.
[10]	WANG Ling-xing, HONG Shan-yan, YANG Mei-li, HUANG Hong-hong. Effects of butylphthalide on microglia activation in frontal lobe of rats after chronic sleep deprivation [J]. CJAP, 2020, 36(2): 106-110.
[11]	WAN Jian-yong, ZHANG Yong, LIU Shi-qiang, HUA Tian-miao, SUN Qing-yan. Effects of exercise intervention on BIM signaling in liver of insulin resistance rats [J]. CJAP, 2020, 36(2): 115-118.
[12]	JIN Zhao, CAO Zheng-tao, WANG Hai-xia, ZHANG Li-hui, LI Yi-feng, ZHOU Yu-bin, WANG Quan, LI Bao-hui, XU Yan, WEI Xiao-yang, WANG Hong, LIN Rong, YANG JING-hui, GENG Xi-chen. Changes of regional cerebral oxygen saturation under gradual-onset rate +Gz exposure [J]. CJAP, 2020, 36(2): 130-133.
[13]	ZHOU Bi-ye, LI Jing, LI Ming-gao, SUN Hai-wen, ZHOU Jia-xing, HAN Lei. Effect of repeated horizontal acceleration exposure on cardiac structure in New Zealand rabbits [J]. CJAP, 2020, 36(2): 134-137.
[14]	YAO Liang-xue, HAN Jia-xin, WEI Pei-yun, HOU Si-bo, HE Xiao-jian, ZHANG Rui-ling, DU Ai-lin. Effects of butylphthalide on H₂S/CBS pathway in hippocampal and amygdala and learning and memory ability in chronic alcoholism rats [J]. CJAP, 2020, 36(2): 138-142.
[15]	LIANG Wan-ling, XIAO Tian-bao, YUAN Feng, CAO Yi-bo, YAN Deng-guo. Effects of microRNA95 targeting FOXD1 gene on radiosensitivity of colorectal cancer loVo cells and its mechanism [J]. CJAP, 2020, 36(2): 148-151.