Effects of Yiqi Huayu Hutan decoction on pulmonary fibrosis in rats and its mechanism

doi:10.12047/j.cjap.5711.2019.023

Abstract

Abstract: Objective: To investigate the effects of Yiqi Huayu Hutan decoction on pulmonary fibrosis of rats which induced by bleomycin. Methods: The rat model of pulmonary fibrosis was induced by intratracheal injection of bleomycin hydrochloride (5 mg/kg). Sixty SD rats were randomly divided into the normal group (group N), the model group (group M), the positive control group (group Y), group of low concentration (group LC), group of medium concentration (group MC) and group of high concentration of Yiqi Huayu Hutan decoction (group HC). After 4 weeks, the experimental groups were treated with low concentration decoction, medium concentration decoction and high concentration decoction respectively, and the Y group was treated with hydrocortisone acetate, the Group N and group M were treated with saline by intragastric administration. Twelve weeks later, rats were killed and the pathomorphism of pulmonary tissues of each group was observed by HE staining and Masson staining. Further, the expressions of transforming growth factor-β1(TGF-β1), Snail1, E-cadherin and Fibronectin in pulmonary tissues of each group were detected by qTR-PCR and Western blot. Results: Compared with the model group, the collagen sediment in the interstitial was reduced in the experimental groups, especially in the group of medium concentration, which was observed by HE staining and Masson staining .Compared with the model group, the expressions of TGF-β1, Snail1 and Fibronectin protein in pulmonary tissues of the treatment groups were decreased in the experimental group, especially in the group of medium concentration, which were detected by qRT-PCR and Western blot. Conclusion: Yiqi Huayu Hutan decoction can significantly improve the pulmonary fibrosis which is induced by bleomycin, and the mechanism is related to the inhibition of the expression of TGF-β/Snail pathway of transcription TGF-β1.

Key words: pulmonary fibrosis, Yiqi Huayu Hutan decoction, TGF-β1, Snail1, rat

TIAN Sheng, CAO Wen-fu, ZHANG Yong-yue, WU Qing. Effects of Yiqi Huayu Hutan decoction on pulmonary fibrosis in rats and its mechanism[J]. CJAP, 2019, 35(2): 101-106.

References

[1] Gong LK, Li XH, Wang H, et al. Effect of Feitai on bleomycin-induced pulmonary fibrosis in rats[J]. J Ethnopharmacol, 2005, 96(3): 537-544.
[2] 刘威, 陈晓玲, 刘建辉, 等. 黄芩苷对博莱霉素诱导的肺纤维化的影响[J]. 中国应用生理学杂志, 2009, 25(2): 145-149.
[3] 陈结慧, 姚文萃, 祝胜郎, 等. 虫草制剂通过TGF-β/Snail信号通路拮抗糖尿病小鼠肾小管上皮细胞间充质转分化[J]. 临床军医杂志, 2017, 45(2): 140-144.
[4] Szapiel SV, Elson NA, Fulmer JD, et al. Bleomycin induced interstitial pulmonary disease in the nude, athymic mouse[J]. Am Rev Respir Dis, 1979, 120(4): 893-899.
[5] 杜丽东, 吴国泰, 王凤玲, 等. 当归黄芪提取物对慢性腹膜功能衰竭大鼠腹膜功能、结构及 TGF-β1 表达的影响[J]. 中国应用生理学杂志, 2016, 32(6): 563-566.
[6] 李江, 黄茂, 武芳, 等. 川芎嗪对博莱霉素致肺纤维化大鼠病理形态学干预作用[J]. 辽宁中医药大学学报, 2008, 10(2): 143-145.
[7] 王璐, 阮彩莲, 杨彦玲, 等. 当归治疗大鼠弥漫性肺间质纤维化的实验研究[J]. 中国应用生理学杂志, 2013, 29(5): 450-452.
[8] 李银生, 牛建昭, 王继峰, 等. 姜黄素对肺纤维化大鼠肺组织胶原沉积及转化生长因子-β1表达的影响[J]. 中华中医药学刊, 2007, 25(1): 55-57.
[9] 宋建平, 田黎, 李瑞琴, 等. 瓜蒌薤白汤对肺纤维化大鼠肺组织及血清中超氧化物歧化酶活力的影响[J]. 河南中医, 2005, 25(8): 22-24.
[10]秦华, 李栋, 曹广生. 海藻多糖对博来霉素诱导的大鼠肺间质纤维化模型的干预作用研究[J]. 齐鲁药事, 2008, 27(9): 554-555.
[11]王宣, 王丽娜, 杨学, 等. 补阳还五汤对肺纤维化大鼠血清HYP含量、肺系数及肺部病理形态学的影响[J]. 时珍国医国药, 2017, 28(7): 1555-1558.
[12]Harris WT, Muhlebach MS, Oster RA, et al. Plasma TGF-beta(1) in pediatric cystic fibrosis: potential biomar-ker of lung disease and response to therapy[J]. Pediatr Pulmonol, 2011, 46(7): 688-695.
[13]Buckley ST, Medina C, Ehrhardt C. Differential susceptibility to epithelial-mesenchymal transition(EMT) of alveolar, bronchial and intestinal epithelial cells in vitro and the effect of angiotensin II receptor inhibition[J]. Cell Tissue Res, 2010, 342(1): 39-51.
[14]庞翠, 张菊, 刘文超, 等. EMT研究进展[J]. 现代肿瘤医学, 2016, 24(15): 2484-2487.
[15]金玉, 张宏文. 苦参碱及氧化苦参碱的抗纤维化作用研究进展[J]. 中国中西医结合肾病杂志, 2004, 5(8): 493-494.