The interventional effects of saturated hydrogen saline on lung injury in rats with cecal ligation and puncture operation

doi:10.12047/j.cjap.5767.2019.024

Abstract

Abstract: Objective: To investigate the hypothesis that hydrogen could ameliorate cecal ligation and puncture (CLP)-induced lung injury of rats by inhibiting cystathionine-gamma-lyase/hydrogen sulfide (CSE/H₂S) system. Methods: A total number of 24 healthy male SD rats weighting 250～300 g were randomly divided into four groups (n=6 in each group): sham operation group(sham group), hydrogen-rich saline control group(H₂ group), CLP group and hydrogen-rich saline treatment group(CLP+H₂ group). The rats were treated with hydrogen-rich saline or saline 10 min before CLP or sham operation. At 8 h of sham or CLP operation, lung samples were obtained to detect the changes of the CSE/H₂S system using biochemical and RT-PCR methods. In order to further confirm the role of H₂S during hydrogen improve the lung injury of CLP rats, we also observed the effect of hydrogen-rich saline on the lung injury induced by H₂S donor-sodium sodium hydrosulfide (NaHS). Thirty-two healthy male SD rats (250~300 g) were randomly divided into four groups (n=8 in each group): control group, H₂S group, H₂S+H₂ group and H₂ group. Saline(10 mg/kg) or NaHS(H₂S donor, 56 μmol/kg) was injected intraperitoneally (10 mg/kg) respectively into rats in the control rats or H₂S group. For rats in the H₂S+H₂ and H₂ group, hydrogen-rich saline (10 mg/kg) was injected 10 min before saline or NaHS administration. Eight hours after the LPS saline or NaHS administration, lung coefficient, MDA content, and MPO activity were detected. The contents of TNF-α, IL-6 and IL-10 in lung tissue were measured, and the morphological changes of lung tissue were also observed. Results: CSE/H₂S system up-regulating were observed in animals exposed to CLP. Hydrogen-rich saline treatment significantly inhibited CSE/H₂S system as indicated by significantly reduced H₂S production in lung, along with a decreased CSE activity and CSE mRNA expression (all P＜0.05). Importantly, the results showed that lung injury and lung tissue inflammation were observed in animals exposed to NaHS. Hydrogen-rich saline treatment significantly attenuated lung injury as indicated by significantly improved histological changes in lung, significantly reduced index of quantitative assessment (IQA), MDA content and lung coefficient (all P＜0.05). MPO activity in lung tissue was significantly reduced along with decreased productions of TNF-α and IL-6, and an increased production of IL-10 in the presence of hydrogen (all P＜0.05), demonstrating antioxidant and anti-inflammatory effect of hydrogen in NaHS-induced ALI. Conclusion: These results indicate that hydrogen-rich saline peritoneal injection improves the lung injury induced by CLP operation. The therapeutic effects of hydrogen-rich saline may be related to suppressing the production of H₂S.

Key words: hydrogen-rich saline, hydrogen sulfide, lung injury, cecal ligation and puncture, rat

ZHAI Yu, ZHOU Xiao-hong, LIU Hui, FU Hong-yi, FAN Ya-min, HUANG Xin-li. The interventional effects of saturated hydrogen saline on lung injury in rats with cecal ligation and puncture operation[J]. CJAP, 2019, 35(2): 107-112.

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