Effects of ginsenoside Rg5 on cell cycle and invasion of gastric cancer

doi:10.12047/j.cjap.5891.2020.011

Abstract

Abstract: Objective: To investigate the effects of ginsenoside Rg5 on the proliferation, cycle and invasion of gastric carcinoma cell lines, providing experimental evidence for the anti-tumor mechanism of ginsenoside Rg5. Methods: In this experimental study, the human immortalized normal gastric mucosa cell GES-1 and gastric adenocarcinoma cell lines AGS and MKN-45 were treated with Rg3 and Rg5 at the concentrations of 10, 20, 30, 40 and 50 μmol/L for 24 h, 3 parallel holes were set for each group. A cell viability test, cell cycle analysis, transwell assay, ELISA and immunoblotting were performed.Results: The viabilities of AGS and MKN-45 were suppressed by Rg3 and Rg5 in a concentration-dependent manner. The activity of Rg5 against gastric cancer cells was stronger than that of Rg3, and its toxicity to GES-1 was lower than that of Rg3. After the treatment of 20 μmol/L of Rg5 for 24 h, Rg5 could generate cell cycle S phase arresting by decreasing the CyclinA1/cyclin-dependent kinase 2 (CDK2)/proliferating cell nuclear antigen (PCNA) complex production and increasing the P21^CIPI. Rg5 inhibited the migration of MNK-45 cells by reducing the expressions of MMP2 and MMP9. WB results showed that Rg5 inhibited the proliferation and migration of gastric cancer mainly by inhibiting the expression of Notch1 protein to regulate its downstream cycle and invasion related proteins.Conclusion: These results suggest that Rg5 exhibits stronger anti-cancer activity than Rg3 in gastric cancer cells, and has higher anti-gastric cancer cell activity than Rg3 and inhibits cell proliferation and migration by regulating the Notch1 pathway.

Key words: Rg5, gastric cancer, cell cycle, cell migration, Notch1

ZHAO Xiang-yu, HE Zhen-yu, ZAI Shou-feng. Effects of ginsenoside Rg5 on cell cycle and invasion of gastric cancer[J]. CJAP, 2020, 36(1): 51-54.

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