Effects of acorus tatarinowii Schott and alpha asarone on free radicals and nNOS/NO in hippocampus of rats with fatigue movement

doi:10.12047/j.cjap.5947.2020.066

Abstract

Abstract: Objective: To study the effects of acorus tatarinowii Schott and its active ingredient-alpha-asarone on learning and memory, free radical metabolism and nNOS/NO signal in hippocampus of rats with fatigue movement. Methods: Eighty SD male rats were randomly divided into eight groups: control group(A), exercise group(B), exercise + alpha-asarone low, middle and high dose treatment group (C, D, E), exercise + acorus tatarinowii Schott low, middle and high dose treatment group (F, G, H),with ten rats in each group. The rats in group C, D and E were administered with alpha-asarone at the doses of 0.10, 0.50 and 1.00 mg.kg^-1.WT^-1 by ig. The rats in group F, G and H were administered with the extracts of Acorus tatarinowii Schott of at the doses of 0.12, 1.20 and 4.80 g.kg^-1.WT^-1by ig. Learning and memory of rats were tested by the method of water maze experiment, and the activities of SOD and NOS, the contents of MDA were detected by the biochemical methods, and the expression levels of nNOS protein in hippocampus of rats were tested by the method of Western blot in at the end of the experiment. Results: The escape latency and MDA content in hippocampus of rats in groups E and H were lower than those in groups B, C, D, F and G and the numbers of Plateau crossing, SOD and NOS activities and the expression levels of nNOS protein in hippocampus of rats were higher than those in groups B, C, D, F and G(P＜0.01). The activities of SOD in hippocampus of rats in groups A, E and H were A＞E＞H, whereas the contents of MDA were opposite (P＜0.01); the activities of NOS and the expression levels of nNOS protein in hippocampus of group E were lower than those of groups A and H (P＜0.01 or P＜0.05), but there was no significant difference between groups A and H (P＞0.05). There were no significant difference in escape latency and numbers of crossing platform among groups A, E and H (P＞0.05). Conclusion: Acorus tatarinowii Schott and alpha-asarone can significantly improve learning and memory of rats with fatigue movement. The mechanism is related to reclaiming the imbalance of free radical metabolism and up-regulating nNOS/NO signal in hippocampus of the rats.

Key words: acorus tatarinowii Schott, alpha-asarone, learning and memory, free radical metabolism, neuronal nitric oxide synthase, fatigue movement, rat

CLC Number:

ZHU Mei-ju, MAO Ze-Hua, GUO Hong-ying, ZHU Hong-zhu, DING Xiao-min. Effects of acorus tatarinowii Schott and alpha asarone on free radicals and nNOS/NO in hippocampus of rats with fatigue movement[J]. CJAP, 2020, 36(4): 306-311.

References

[1] 朱梅菊, 谭宁华, 陈斌, 等.石菖蒲等七味中药体内抗运动性疲劳的实验研究[J].湘潭师范学院学报, 2009, 31(4) : 38-40.
[2] 卢成淑, 冯宁, 南国, 等. 石菖蒲及其活性成分防治阿尔茨海默病的研究进展[J]. 中草药, 2016, 47(7): 1236-1242.
[3] 李志勇, 赵晖, 张琪. 两种血管性痴呆模型大鼠学习记忆能力与海马自由基代谢的比较[J]. 中国老年学杂志, 2012, 32(22): 4940-4942.
[4] Yu SY, Gao R, Zhang L, et al. Curcumin ameliorates ethanol-induced memory deficits and enhanced brain nitric oxide synthase activity in mice[J]. Prog Neuropsychopharmacol Biol Psychiatry, 2013, 44: 210-216.
[5] 贺石林.中医科研设计与统计方法[M]. 长沙: 湖南科学技术出版社, 1988: 55-66.
[6] 孙丽娜, 刘晓莉, 乔德才. 运动疲劳可能通过激活海马小胶质细胞介导的神经炎症降低大鼠学习记忆功能[J]. 天津体育学院学报, 2014, 29(3): 193-196.
[7] 朱洪竹, 张莹, 朱梅菊,等. 螺旋藻对运动疲劳大鼠海马损伤的保护作用及机制研究[J]. 中国应用生理学杂志, 2018, 34(6): 562-567.
[8] 陈惠花, 朱梅菊, 朱洪竹, 等. 石菖蒲及其活性成分-5-羟甲基糠醛对疲劳运动大鼠学习记忆的影响及其机制[J]. 中国应用生理学杂志, 2019, 35(4): 366-371.
[9] 王光明, 李绍林, 蔡琳, 等. 石菖蒲挥发油微乳对慢性应激小鼠记忆的影响及其机理研究[J]. 新中医, 2017, 49(12): 4-7.
[10] 黄小燕, 陈希, 张伟雯, 等. α-细辛醚对Fmr1基因敲除小鼠跳台行为和海马p-Akt 表达的影响[J]. 临床和实验医学杂志, 2016, 15(17): 1661-1665.
[11] 代朋乙, 黄昌林. 运动性疲劳研究进展[J]. 解放军医学杂志, 2016, 41(11): 955-964.
[12] 孙竹梅, 赵雅宁, 李建民, 等. 不同强度运动对脑缺血再灌注大鼠学习能力及氧自由基代谢的影响[J]. 中国康复理论与实践, 2015, 21(1): 26-30.
[13] Barp CG, Mendes C, Lemos-senna E, et al. 7-nitroindazol-loaded nanoemulsions: Preparation, characterization and its improved inhibitory effect on nitric oxide synthase-1[J]. Nitric Oxide, 2018, 76: 129-135.
[14] 张焕, 苑韬, 时艳杰, 等. 醒脑静对麻醉后老年认知功能障碍大鼠学习记忆及海马区nNOS、血清β-淀粉样蛋白的影响[J]. 辽宁中医药大学学报, 2016, 18(6): 5-7.
[15] Gocmez SS, Yazir Y, Sahin D, et al. The effect of a selective neuronal nitric oxide synthase inhibitor 3-bromo 7-nitroindazole on spatial learning and memory in rats[J]. Pharmacol Biochem Behav, 2015, 131: 19-25.
[16] Moosavli M, Abbasi L, Zarifkar A, et al. The role of nitric oxide in spatial memory stages, hippocampal ERK and CaMKII phosphorylation[J]. Pharmacol Biochem Behav, 2014, 122: 164-172.
[17] Matsumoto Y, Matsumoto CS, Takahashi T, et al. Activation of NO-cGMP signaling rescues age-related memory impairment in crickets[J]. Front Behav Neurosci, 2016, 10: 166-176.
[18] 高婧玮, 虞立霞, 洪燕, 等. 转人P301L突变tau基因小鼠学习记忆功能与脑内NO的改变[J]. 中国应用生理学杂志, 2015, 31(5): 385-389.
[19] 李峰, 杨维益, 季绍良, 等. 运动性疲劳大鼠脑组织中一氧化氮合酶和内皮素mRNA表达的变化规律及中药“体复康”的调节作用[J]. 中国运动医学杂志, 2001, 20(2): 116-120.
[20] 刘鸿宇, 刘定一, 杨桂姣, 等. 运动性疲劳对大鼠杏仁体一氧化氮合酶表达的影响[J]. 体育科学, 2005, 25(2): 79-81.
[21] 张智华, 王秀莲,吴建红, 等. 石菖蒲-远志药对不同组分对AD模型大鼠学习记忆和海马突触蛋白表达的影响[J]. 中国实验方剂学杂志, 2016, 22(7): 120-124.
[22] 景玉宏, 冯慎远, 汤晓琴. 菖蒲对学习记忆的影响及突触机制[J]. 中国中医基础医学杂志, 2002, 8(6): 38-40.