Home  About Journal Instructions for Authors Editorial Board Subscribe Advertisement Messages Board Contact Us 中文

CJAP ›› 2020, Vol. 36 ›› Issue (4): 346-349.doi: 10.12047/j.cjap.5922.2020.074

• ORIGINAL ARTICLES • Previous Articles     Next Articles

Astragalus injection mediates autophagy and myocardial remodeling in rats with ischemic cardiomyopathy through calumenin

SHAO Li-qun, BAI Ting-ting1, ZHAO Qiang1, WANG Chun-gui2, XUAN Li-ying2, WANG Yu2△, ZHAO Ming1△   

  1. 1. Affiliated Hospital of Inner Mongolia University for National,
    2. Medical College of Inner Mongolia University for Nationalities, Tongliao 028000, China
  • Received:2019-08-06 Revised:2020-05-14 Published:2020-11-09

Abstract: Objective: To study the effects of astragalus injection on myocardial remodeling, calumenin and autophagy in rats with ischemic cardiomyopathy.Methods: Thirty-six male SD rats were divided into normal control group, ischemic cardiomyopathy group and astragalus injection group, 12 in each group. Electrocardiogram (ECG) and echocardiography were performed before operation in three groups. Rats in ischemic cardiomyopathy group and astragalus injection group underwent thoracotomy and ligation of coronary artery for 20 minutes, then thoracic cavity was closed after reperfusion. In the astragalus injection group,10 g/kg body weight of Astragalus injection was injected once a week, four times in total. Four weeks after operation, rats in three groups were executed by echocardiography and their hearts were collected for Hematoxylin-Eosin (HE) staining and Van Gieson (VG) staining to observe myocardial pathological changes. Calumenin, LC3-I, LC3-II expressions and LC3-I/LC3-II ratio were detected by Western blot.Results: Compared with ischemic cardiomyopathy group, the echocardiography and myocardial pathology of rats in astragalus injection group changed obviously, and the expressions of calumenin, LC3-I, LC3-II and LC3-I/LC3-II ratio changed significantly (P<0.01).Conclusion: Astragalus injection has apparent inhibitory effect on ventricular remodeling and autophagy of myocardial cells in rats with ischemic cardiomyopathy, which may be mediated by calumenin.

Key words: astragalus injection, ischemic cardiomyopathy, myocardial remodeling, calumenin, autophagy, rat

CLC Number: 

Copyright © 2015 CJAP, All Rights Reserved.
Powered by Beijing Magtech Co. Ltd