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CJAP ›› 2020, Vol. 36 ›› Issue (4): 358-362.doi: 10.12047/j.cjap.5940.2020.077

• ORIGINAL ARTICLES • Previous Articles     Next Articles

Expression of ANO1 during cardiac fibroblasts differentiation and its electrophysiological characteristics as calcium-activated chloride channel protein

TIAN Xiang-qin1,2, TAN Chao-yang1,3, LI Na-na2, CHANG Yu-qiao2, ZHANG Ai-mei4, ZHOU Ying1, MA Ke-tao1, SI Jun-qiang   

  1. 1. Department of Physiology, Medical College of Shihezi University, Shihezi 832002;
    2. Key Open Laboratory for Tissue Regeneration of Henan Province, Xinxiang Medical University, Xinxiang 453003;
    3. Karamay Army Division of Chinese People's Liberation Army, Karamay 834000;
    4. First Affiliated Hospital, Medical College of Shihezi University, Shihezi 832002, China
  • Received:2019-09-18 Revised:2020-04-17 Published:2020-11-09

Abstract: Objective: To investigate the expression and electrophysiological characteristics of calcium-activated chlorine channel anoctamin-1 (ANO1) protein during the differentiation of cardiac fibroblasts (CFs) into myofibroblasts (MFs), and to elucidate the role of ANO1 in myocardial fibrosis. Methods: The primary CFs from neonatal rats were isolated and the cells differentiated into MFs by subculture. The Ca2+-activated Cl- current (ICl(Ca)) in CFs and MFs were measured by whole-cell patch clamp, and the expressions of ANO1, α-smooth muscle actin(α-SMA)and vimentin in CFs and MFs were detected by immunofluorescence assay and Western blot, respectively. Results: The current density in the early adherent CFs was stronger than that in MFs. ANO1 was expressed preferentially within and around the nuclei, and a small amount of ANO1 was expressed on the cell membrane. Moreover, ANO1 expression was weak in the early adherent CFs and displayed stronger expression in the MFs with proliferation tendency. Conclusion: The expression of ANO1 is closely related to the differentiation of MFs and it may be involved in modulation myocardial fibrosis.

Key words: cardiac fibroblasts, calcium-activated chloride channels, ANO1, whole-cell patch clamp, myocardial fibrosis

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