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CJAP ›› 2020, Vol. 36 ›› Issue (5): 444-448.doi: 10.12047/j.cjap.5939.2020.094

• ORIGINAL ARTICLES • Previous Articles     Next Articles

Effects of Ganmai Dazao Tang on synaptic structure of hippocampal neurons in depression model rats

GUO Rui1△, QIN Wei-shuai1, ZHANG Shuang-yong1, ZHANG Shu-ling2   

  1. 1. Department of Encephalopathy, Zhengzhou Chinese Medicine Hospital, Zhengzhou 450007;
    2. Department of Neurology, Zhengzhou People 's Hospital, Zhengzhou 450003, China
  • Received:2019-09-10 Revised:2020-06-16 Published:2021-02-25

Abstract: Objective: To investigate the effects of Ganmai Dazao Tang on behavior and monoamine neurotransmitters in rats with depression, and to explore its potential mechanism from synaptic structure. Methods: Sixty SD rats were randomly divided into 5 groups: normal control group, model group, fluoxetine group (10.8 mg/kg), Ganmai Dazao Tang high and low dose group (9.72, 4.86 g/kg), 12 rats in each group. Except the control group, the rats in the other groups were all chronically unpredictable mild stress (CUMS) to establish a depression model, and were treated by intragastric administration for 21 days. The depression-like behaviors of rats were evaluated by sucrose consumption test and open field test. The contents of serotonin (5-HT) and norepinephrine (NE) in hippocampus were detected by ELISA. The synaptic damage of neurons was observed by Golgi staining. The synaptic structure protein expression levels of MAP-2 and GAP-43 of hippocampus were detected by Immunohistochemistry and Western blot. Results: Compared with control group, the sucrose preference and autonomic activity scores of the depression model rats were decreased significantly (P<0.01), the levels of 5-HT and NE in hippocampus were decreased significantly (P<0.01), and the dendritic spines were absent, and the expressions of MAP-2 and GAP-43 were down-regulated significantly (P<0.01). After treated with Ganmai Dazao Tang, the depression-like behavior of the model rats was significantly relieved (P<0.01), and the levels of 5-HT and NE were increased (P< 0.05). Dendritic spine density, length and branching were increased, the expressions of MAP-2 and GAP-43 were increased (P< 0.01). Conclusion: Ganmai Dazao Tang can improve the depression-like behavior of depression model rats and increase the monoamine neurotransmitter content in hippocampus, which may be related to up-regulation of synaptic structural proteins and relief of synaptic damage in neurons.

Key words: Ganmai Dazao Tang, depression, hippocampus, synapse, rat

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