Effect of nuclear radiation on rat model of decompression sickness induced by large depth rapid floating escape

doi:10.12047/j.cjap.6086.2021.059

Abstract

Abstract: Objective: To investigate the effects of different doses of nuclei exposure at different time on morbidity, mortality, and damage indicators in a rat model of decompression sickness caused by rapid flotation escape at a large depth. Methods: Eighty male SD rats were randomly divided into blank control group, escape control group and six intervention groups (escape at 4 hours after 4 Gy radiation, escape at 4 hours after 6 Gy radiation, escape at 4 hours after 12 Gy radiation, escape at 8 hours after 4 Gy radiation, escape at 8 hours after 6 Gy radiation, escape at 8 hours after 12 Gy radiation). Rats in intervention groups were exposed to different doses of γ-ray (4,6,12 Gy, respectively), and then were carried out a large depth and rapid buoyancy escape experiment (maximum pressure depth of 150 m). The changes of lung W/D, spleen index and plasma IL-1β levels were analyzed. Results: Compared with the blank control group, decompression sickness incidence and mortality of rats in escape groups after nuclear exposure were increased significantly. In 4 Gy and 6 Gy irradiation groups, higher morbidity and mortality were observed in rats which escaped at 4 h post nuclear exposure when compared with rats in 8 h groups. Consistent with the changes in morbidity and mortality, the wet / dry ratio of lung tissue, the pathological damage of lung tissue, and the decrease of spleen index showed the same trends: the changes were obvious at 4 h after lower doses nuclear radiation (4 Gy and 6 Gy), not at 8 h. However, these indicators all changed markedly at 4 and 8 h after higher doses nuclear radiation (12 Gy). Plasma IL-1β levels were significantly increased in each post-radiation exposure group when compared with the blank control group and the exposed control group. Conclusion: Nuclear radiation-induced lung injury, the damaged immune function and elevated plasma inflammatory factor concentrations increase the risk of decompression sickness after rapid ascent.

Key words: nuclear radiation, rapid floating escape, decompression sickness, lung injury, inflammatory factors, rats

CLC Number:

R847

XU Ji, FANG Yi-qun, BAO Xiao-chen, YUAN Heng-rong, WANG Nan, WANG Fang-fang. Effect of nuclear radiation on rat model of decompression sickness induced by large depth rapid floating escape[J]. CJAP, 2021, 37(5): 486-489.

References

[1] 王月兴, 贾福星, 马晓琳, 等. 核潜艇核事故艇内放射性应急防护和中子剂量快速评价研究[J]. 中华航海医学与高气压医学杂志, 2005, 12(4): 193-196.
[2] 王川, 张建国, 王月兴. 核潜艇海上核事故医学应急救援研究[J]. 原子能科学技术, 2012, 46(9): 689-693.
[3] 刘畅, 王仁生. 核潜艇核事故医学应急救援[J]. 辐射防护通讯, 2018, 38(5): 28-31.
[4] Joel E, Geoffrey L, Fiona S, et al. Prediction of signs/symptoms of decompression sickness following submarine tower escape[J]. Undersea Hyperb Med, 2019, 46(1): 17-33.
[5] Wang HT, Fang YQ, You P, et al. PDTC ameliorates decompression induced-lung injury caused by unsafe fast buoyancy ascent escape via inhibition of NF-κB pathway[J]. Undersea Hyperb Med, 2018, 45(3): 351-362.
[6] 陈锐勇, 廖昌波, 姚健, 等. 快速上浮脱险装备性能试验极限安全深度的理论推算[J]. 海军医学杂志, 2019, 40(6): 501-505.
[7] 许骥, 何佳, 陈锐勇, 等. 模拟大深度快速上浮脱险兔血浆血管性血友病因子和一氧化氮水平变化[J]. 微循环学杂志, 2015, 25(4): 36-38.
[8] Wang HT, Fang YQ, Bao XC, et al. Expression changes of TNF-α, IL-1β and IL-6 in the rat lung of decompression sickness induced by fast buoyancy ascent escape[J]. Undersea Hyperb Med, 2015, 42(1): 23-31.
[9] 刘倩倩, 刘海宽, 陈波, 等. Voxel体素模型在辐射防护的应用和进展[J]. 中国医学物理学杂志, 2015, 32(5): 629-634.
[10] 仪丽荣, 田梅, 朴春南, 等. 丙二醇对预防 5.5 Gy ⁶⁰Co γ射线全身照射小鼠辐射损伤的初步探讨[J]. 中国辐射卫生, 2019, 28(1): 17-21.
[11] 付青姐, 李明春, 柳迎华. 辐射对小鼠脾脏损伤及香菇多糖的保护作用[J]. 中国现代应用药学, 2013, 30(6): 606-609.
[12] 于涛, 王蕾, 纪建达, 等. 两种海洋生物多糖对⁶⁰Co辐射损伤小鼠保护作用的研究[J]. 辐射防护, 2019, 39(3): 242-248.
[13] 张梦欣, 张杰森, 李勇森, 等. 甘草甜素对C57BL /6小鼠脾脏中DC和巨噬细胞及其细胞因子分泌的影响[J]. 现代免疫学, 2019, 39(5): 397-401.
[14] 董广通, 张解玉, 侯炜. 放射性肺损伤的防治研究进展[J]. 中国医药导报, 2018, 15(30): 24-27.
[15] 张师, 包晓辰, 方以群, 等. 模拟大深度潜艇艇员快速上浮脱险致减压病大鼠模型的建立[J]. 中华航海与高气压医学杂志, 2012, 19(5): 284-287.
[16] Oh SH, Kang HD, Jung SK, et al. Successful treatment of decompression sickness complicated with acute hepatic infarction and acute kidney injury: a case report[J]. Undersea Hyperb Med, 2019, 46(1): 81-85.
[17] 王楠, 毕珂, 包晓辰, 等. 减压病大鼠模型心脏血流动力学的改变 [J]. 军事医学, 2020, 44(5): 345-348.
[18] Yang K, Palm J, Konig J, et al. Matrix-Metallo-Proteinases and their tissue inhibitors in radiation-induced lung injury[J]. Int J Radiat Biol, 2007, 83(10): 665-676.
[19] Sohn SH, Lee JM, Park S, et al. The inflammasome accelerates radiation-induced lung inflammation and fibrosis in mice[J]. Environ Toxicol Pharmacol, 2015, 39(2): 917-926.
[20] 束明慧, 唐品升, 左万里, 等. 放射剂量对大鼠放射性肺损伤的影响[J]. 江苏医药, 2019, 45(12): 1197-封2.
[21] 张志权, 孙玲玲. IL-1β与IL-18在急性肺损伤中的表达及临床意义[J]. 海南医学, 2017, 28(12): 1954-1956.