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CJAP ›› 2017, Vol. 33 ›› Issue (1): 51-55.doi: 10.12047/j.cjap.5448.2017.012

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Effects of Shiquanyuzhentang on immunologic function of H22 tumor-bearing mouse

ZHU Yun-chao, ZHAN Guang-jie, YUAN De-pei, YANG Fu-ming, HUANG Qiong   

  1. School of Medical Science, HuBei University for Nationalities, Enshi 445000, China
  • Received:2015-11-17 Revised:2016-10-25 Online:2017-01-28 Published:2018-06-19
  • Supported by:
    国家中医药管理局公共卫生专项资金项目(财社[2010]91号);湖北民族学院博士科研启动基金项目(MY2010B004)

Abstract: Objective: To investigate the effects of Shiquanyuzhentang (Traditional Chinese Medicine) on the immunologic function of tu-mor-bearing mice and its mechanism of antitumor.Methods: Thirty SPF grade male Kunming mice transplanted with H22 hepatocellular carci-noma were divided into three groups randomly:model group,positive control group and Shiquanyuzhentang group(n=10). Another 10 mice were selected as normal control group. Normal control group and model group received normal saline and distilled water supplementation by 10 mL/kg everyday. Positive control group and Shiquanyuzhentang group received Shengyi(80 mg/ml)and Shiquanyuzhentang decoction at the doses of 8 g/kg and 18 g/kg respectively everyday. After 14 days of continuous administration, the mice were killed and the thymus, spleen index, tumor inhibition rate,peripheral blood leukocytes,lymphocyte content,cell percentage of T cell subsets CD3, CD4, CD8,interleukin 2 (IL-2)in serum,tumor necrosis factor-α(TNF-α),interferon β(IFN-β) content,lymphocyte proliferation ability and NK cell were measured.Results: Compared with normal control group, the weight of mice and thymus and spleen index of the Shiquanyuzhentang group were increased significantly(P<0.05);leukocyte and lymphocyte CD3、CD4、CD8 and TNF-α were increased greatly(P<0. 05),IL-2 and IFN-β were de-creased significantly(P<0.05). Compared with model group,thymus and spleen index of Shiquanyuzhentang group were increased significant-ly(P<0.05);leukocyte and lymphocyte CD3、CD4、IL-2、IFN-β and TNF-α of Shiquanyuzhentang group were increased significantly(P<0. 05),CD8 content was decreased significantly(P<0.05);NK cell and lymphocyte proliferation were increased significantly(P<0.05).Conclusion: Shiquanyuzhentang can promote the growth of immune organs in mice bearing H22, enhance immune function and is beneficial to the recovery of tumor body.

Key words: Shiquanyuzhentang, H22 tumor-bearing mouse, immunity

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